Inhibitory effects of phenolic glycosides from Trollius chinensis Bunge on α-glucosidase: inhibition kinetics and mechanisms.
Food Funct
; 13(5): 2857-2864, 2022 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-35179535
ABSTRACT
Two undescribed phenolic glycosides, trochinenols B and C (1 and2), together with four known analogues (3-6), were isolated from the functional tea Trollius chinensis Bunge and their α-glucosidase inhibitory kinetics and mechanisms were investigated. It was found that 1 inhibited α-glucosidase in a noncompetitive manner with an IC50 value of 25.96 µM, while 3 showed a notable inhibitory effect against α-glucosidase in an uncompetitive manner with an IC50 value of 3.14 µM. Analysis of synchronous fluorescence and circular dichroism spectroscopy indicated that the binding of 1 to α-glucosidase led to the rearrangement and conformational alteration of the α-glucosidase enzyme. Furthermore, molecular docking indicated that 1 had a high affinity close to the active site pocket of α-glucosidase and indirectly inhibited the catalytic activity of the enzyme. However, 3 was bound to the entrance part of the active center of α-glucosidase and could hinder the release of the substrate as well as the catalytic reaction product, eventually suppressing the catalytic activity of α-glucosidase.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenóis
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Extratos Vegetais
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Ranunculaceae
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Alfa-Glucosidases
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Inibidores de Glicosídeo Hidrolases
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Glicosídeos
Idioma:
En
Revista:
Food Funct
Ano de publicação:
2022
Tipo de documento:
Article