Electron probe analysis, X-ray mapping, and electron energy-loss spectroscopy of calcium, magnesium, and monovalent ions in log-phase and in dividing Escherichia coli B cells.
J Bacteriol
; 167(3): 935-9, 1986 Sep.
Article
em En
| MEDLINE
| ID: mdl-3528130
ABSTRACT
The elemental composition of individual cells of rapidly frozen and cryosectioned Escherichia coli B was measured with electron optical microanalytic methods. The Ca content was high (26.2 mmol/kg) in a 10-nm-wide region of the cell envelope. Amounts of cytoplasmic Ca in actively dividing cells were significantly higher (32.6 mmol/kg [dry weight]) than in the log-phase (1.5 mmol/kg) cells. Cellular Mg was 205 mmol/kg (dry weight) and it was uniformly distributed throughout the cell. Cells washed in distilled water before freezing lost monovalent ions (Na, Cl, and K), but the membrane-bound Ca and cellular Mg were not reduced, indicating that cellular Mg and membrane Ca are more tightly bound.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Escherichia coli
/
Magnésio
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1986
Tipo de documento:
Article