A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of plum viroid I (PlVd-I).
J Virol Methods
; 306: 114543, 2022 08.
Article
em En
| MEDLINE
| ID: mdl-35580684
ABSTRACT
Plum viroid I (PlVd-I) is found in marbling and corky flesh diseased plum trees in South Africa. In this study a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the high-throughput detection of PlVd-I was developed. This assay can be performed on crude extracts and detection can either be a pH dependent colorimetric reaction or a real-time fluorescent signal reaction. The false discovery rate was shown to be low and no decrease in sensitivity was detected compared to RT-PCR. The RT-LAMP assay allows for the fast and cost-effective detection of PlVd-I that will curtail the distribution of infected plant material.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Viroides
/
Prunus domestica
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
África do Sul