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Key Glycosyltransferase Genes of Panax notoginseng: Identification and Engineering Yeast Construction of Rare Ginsenosides.
Jiang, Zhouqian; Gao, Haiyun; Liu, Rong; Xia, Meng; Lu, Yun; Wang, Jiadian; Chen, Xiaochao; Zhang, Yifeng; Li, Dan; Tong, Yuru; Liu, Panting; Liu, Yuan; Luo, Yunfeng; Gao, Jie; Yin, Yan; Huang, Luqi; Gao, Wei.
Afiliação
  • Jiang Z; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Gao H; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Liu R; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Xia M; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Lu Y; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Wang J; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Chen X; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Zhang Y; Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, People's Republic of China.
  • Li D; School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, People's Republic of China.
  • Tong Y; School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, People's Republic of China.
  • Liu P; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Liu Y; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Luo Y; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Gao J; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
  • Yin Y; School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, People's Republic of China.
  • Huang L; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, People's Republic of China.
  • Gao W; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, People's Republic of China.
ACS Synth Biol ; 11(7): 2394-2404, 2022 07 15.
Article em En | MEDLINE | ID: mdl-35687875
Panax notoginseng is one of the most famous valuable medical plants in China, and its broad application in clinical treatment has an inseparable relationship with the active molecules, ginsenosides. Ginsenosides are glycoside compounds that have varied structures for the diverse sugar chain. Although extensive work has been done, there are still unknown steps in the biosynthetic pathway of ginsenosides. Here, we screened candidate glycosyltransferase genes based on the previous genome and transcriptome data of P. notoginseng and cloned the full length of 27 UGT genes successfully. Among them, we found that PnUGT33 could catalyze different ginsenoside substrates to produce higher polarity rare ginsenosides by extending the sugar chain. We further analyzed the enzymatic kinetics and predicted the catalytic mechanism of PnUGT33 by simulating molecular docking. After that, we reconstructed the biosynthetic pathway of rare ginsenoside Rg3 and gypenoside LXXV in yeast. By combining the Golden Gate method and overexpressing the UDPG biosynthetic genes, we further improved the yield of engineering yeast strain. Finally, the shake-flask culture yield of Rg3 reached 51 mg/L and the fed-batch fermentation yield of gypenoside LXXV reached 94.5 mg/L, which was the first and highest record.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ginsenosídeos / Panax notoginseng / Panax Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Synth Biol Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ginsenosídeos / Panax notoginseng / Panax Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Synth Biol Ano de publicação: 2022 Tipo de documento: Article