Effects of electrolysed water and levulinic acid combination on microbial safety and polysaccharide nanostructure of organic strawberry.

This study aimed to better understand the effects of acidic electrolysed water (AEW, 4 mg/L) and levulinic acid (LA, food grade, 2%) combination on organic strawberry over 7 days. This combined method reduced the population of strawberry's natural microbiota by 1-2 log CFU/g and kept the level of inoculated Escherichia coli O157:H7 and Salmonella below the detection limit (2 log CFU/g) during the whole storage period. Meanwhile, AEW + LA did not affect the physicochemical qualities of strawberries significantly, maintaining most texture and biochemical attributes at an acceptable level (e.g., firmness, colour, soluble solids content and organic acid content). Atomic force microscopy further revealed that the treatment containing LA preserved the sodium carbonate soluble pectin (SSP) nanostructure best by maintaining their length and height, and slowed the breakdown of SSP chains by promoting acid-induced bonding and soluble pectin precipitation. These results demonstrated that low concentration AEW and LA combination is a promising sanitising approach for organic strawberry preservation.