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N-3 polyunsaturated fatty acids block the trimethylamine-N-oxide- ACE2- TMPRSS2 cascade to inhibit the infection of human endothelial progenitor cells by SARS-CoV-2.
Chiang, En-Pei Isabel; Syu, Jia-Ning; Hung, Hung-Chang; Rodriguez, Raymond L; Wang, Wei-Jan; Chiang, En-Rung; Chiu, Shao-Chih; Chao, Che-Yi; Tang, Feng-Yao.
Afiliação
  • Chiang EI; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, Republic of China; Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, Taiwan, Republic of China.
  • Syu JN; Biomedical Science Laboratory, Department of Nutrition, China Medical University, Taichung, Taiwan, Republic of China.
  • Hung HC; Department of Internal Medicine, Nantou Hospital, Ministry of Health and Welfare, Nantou City, Taiwan, Republic of China.
  • Rodriguez RL; Department of Molecular and Cellular Biology, University of California, Davis, California, USA.
  • Wang WJ; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, Republic of China.
  • Chiang ER; Department of Orthopedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China; National Yang Ming Chiao Tung University, School of Medicine, Taipei, Taiwan, Republic of China.
  • Chiu SC; Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, Republic of China; Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, Republic of China.
  • Chao CY; Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, Republic of China; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, Republic of China.
  • Tang FY; Biomedical Science Laboratory, Department of Nutrition, China Medical University, Taichung, Taiwan, Republic of China. Electronic address: vincenttang@mail.cmu.edu.tw.
J Nutr Biochem ; 109: 109102, 2022 11.
Article em En | MEDLINE | ID: mdl-35817244
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) is a novel coronavirus that infects many types of cells and causes cytokine storms, excessive inflammation, acute respiratory distress to induce failure of respiratory system and other critical organs. In this study, our results showed that trimethylamine-N-oxide (TMAO), a metabolite generated by gut microbiota, acts as a regulatory mediator to enhance the inerleukin-6 (IL-6) cytokine production and the infection of human endothelial progenitor cells (hEPCs) by SARS-CoV-2. Treatment of N-3 polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) could effectively block the entry of SARS-CoV-2 in hEPCs. The anti-infection effects of N-3 PUFAs were associated with the inactivation of NF-κB signaling pathway, a decreased expression of the entry receptor angiotensin-converting enzyme 2 (ACE2) and downstream transmembrane serine protease 2 in hEPCs upon the stimulation of TMAO. Treatment of DHA and EPA further effectively inhibited TMAO-mediated expression of IL-6 protein, probably through an inactivation of MAPK/p38/JNK signaling cascades and a downregulation of microRNA (miR)-221 in hEPCs. In conclusion, N-3 PUFAs such as DHA and EPA could effectively act as preventive agents to block the infection of SARS-CoV-2 and IL-6 cytokine production in hEPCs upon the stimulation of TMAO.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Graxos Ômega-3 / MicroRNAs / Células Progenitoras Endoteliais / COVID-19 Idioma: En Revista: J Nutr Biochem Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Graxos Ômega-3 / MicroRNAs / Células Progenitoras Endoteliais / COVID-19 Idioma: En Revista: J Nutr Biochem Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China