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Qilan preparation inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells.
Jiamin, Ding; Yifeng, Xing; Zuoliang, Chen; Wanlu, Chen; Zhongxiong, M A; Yunde, Xie; Lin, Zhou.
Afiliação
  • Jiamin D; Department of Oral Mucosal Diseases, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China.
  • Yifeng X; School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
  • Zuoliang C; Department of Oral Mucosal Diseases, Xiamen Stomatological Hospital, School of Stomatology, Fujian Medical University, Xiamen 361003, China.
  • Wanlu C; Department of Oral Mucosal Diseases, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China.
  • Zhongxiong MA; Department of Oral Mucosal Diseases, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China.
  • Yunde X; Department of Oral Mucosal Diseases, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China.
  • Lin Z; Department of Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350001, China.
J Tradit Chin Med ; 42(5): 693-700, 2022 10.
Article em En | MEDLINE | ID: mdl-36083475
OBJECTIVE: The aim of this study was to examine the antitumor effects of Qilan preparation on oral squamous cell carcinoma (OSCC) and to investigate its underlying mechanisms of action. METHODS: Cell proliferation, cell cycle distribution and apoptosis were examined using cell counting kit-8 (CCK8) and flow cytometry (FCM). The expression of PTEN and PDCD4 were determined by western blot. Changes in miR-21 levels were quantified using TaqMan stem-loop real-time PCR. After miR-21 was transiently transfected into Tca8113 cells using Lipofectamine®3000, cell proliferation, apoptosis and miR-21 and PDCD4 expression levels were measured. RESULTS: Qilan preparation inhibited Tca8113 cell growth in a dose- and time-dependent manner by inducing apoptosis and cell cycle arrest in S-phase, decreasing miR-21 levels and increasing PTEN and PDCD4 expression. MiR-21 overexpression reversed the Qilan preparation-induced suppression of cell proliferation and induction of apoptosis while also blocking the increase in PDCD4. CONCLUSIONS: Our study revealed, for the first time, the ability of Qilan preparation to suppress TSCC cell growth and elucidated that Qilan preparation elicits its anti-cancer actions either the miR-21/PDCD4 or PTEN pathway.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Neoplasias da Língua / Carcinoma de Células Escamosas / MicroRNAs Idioma: En Revista: J Tradit Chin Med Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Neoplasias da Língua / Carcinoma de Células Escamosas / MicroRNAs Idioma: En Revista: J Tradit Chin Med Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China