An enzymatic colorimetric whole-cell biosensor for high-throughput identification of lysine overproducers.
Biosens Bioelectron
; 216: 114681, 2022 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-36087402
ABSTRACT
L-lysine is a crucial nutrient for both humans and animals, and its main commercial use is as a supplement in animal feed to promote chicken and other animal growth. Fluorescence biosensors based on the transcriptional regulator have been developed for high-throughput screening of L-lysine producers. However, due to its inability to specifically detect lysine, this fluorescent biosensor cannot be employed to screen high-yielding strains. Here, we present a novel technique for observing L-lysine concentrations within individual Corynebacterium glutamicum cells. The transcriptional regulator LysG and its binding site, as well as the phytoene desaturase that catalyzes the synthesis of the red pigment, make up the functional core of the biosensor. The lysine-sensitive mutant LysG(E123Y, E125A), which improved the sensitivity of biosensors, was generated by site-directed saturation mutagenesis. In addition, we increased the lysine-induced chromogenic biosensor response to 320 mM by optimizing the L-lysine export mechanism and the pathway for the synthesis of lycopene precursors. The direct identification of producers with elevated L-lysine accumulation is thus made straightforward by colorimetric screening. Lys-8, a lysine producer with a maximum lysine titer of 316.2 mM, was sorted out based on the biosensor. The enzymatic colorimetric biosensor constructed here is a simple tool with great potential for the development of high-level lysine-producing C. glutamicum.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Corynebacterium glutamicum
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biosens Bioelectron
Ano de publicação:
2022
Tipo de documento:
Article