Whole genome analysis of Enterobacter cloacae Rs-2 and screening of genes related to plant-growth promotion.
Environ Sci Pollut Res Int
; 30(8): 21548-21564, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36272007
ABSTRACT
The genus Enterobacter is widely recognized for its biotechnology potential in improving soil environment and crop growth promotion. To further explore these biotechnological potentials, we sequenced and analyzed the whole genome of Enterobacter cloacae Rs-2. The analysis showed that the total length of the Rs-2 genome was 6,965,070,514 bp, and GC content was 55.80%; the annotation results of GO and COG databases showed that the genome contains a variety of growth-promoting genes, such as iscU, glnA, glnB (nitrogen fixation); iucABCD (siderophore synthesis) and fepA, fcuA, fhuA, and pfeA, etc. (siderophore transport); ipdC (secreted IAA) and gcd, pqqBCDEF (dissolved phosphorus), etc. No pathogenic factors such as virulence genes were found. The application of Rs-2 as a soil inoculant in pot experiments showed great potential for growth promotion. This study proved the plant growth-promoting ability of Rs-2 at the molecular level through genetic screening and analysis, which provided guidance for the further improvement of the strain and laid a foundation for its application in agricultural production.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Enterobacter cloacae
/
Sideróforos
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Environ Sci Pollut Res Int
Ano de publicação:
2023
Tipo de documento:
Article