Controlled Production of Carnosic Acid and Carnosol in Cell Suspensions of Lepechinia meyenii Treated with Different Elicitors and Biosynthetic Precursors.
Chem Biodivers
; 20(2): e202200733, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36562957
ABSTRACT
Lepechinia meyenii is a medicinal plant specialized in the biosynthesis of different types of antioxidants including the diterpenes carnosic (CA) acid and carnosol (CS). Herein we present the results of plant tissue culture approaches performed in this medicinal plant with particular emphasis on the generation and evaluation of a cell suspension system for CA and CS production. The effect of sucrose concentration, temperature, pH, and UV-light exposure was explored. In addition, diverse concentrations of microbial elicitors (salicylic acid, pyocyanin, Glucanex, and chitin), simulators of abiotic elicitors (polyethylene glycol and NaCl), and biosynthetic precursors (mevalonolactone, geranylgeraniol, and miltiradiene/abietatriene) were evaluated on batch cultures for 20â
days. Miltiradiene/abietatriene obtainment was achieved through a metabolic engineering approach using a recombinant strain of Saccharomyces cerevisiae. Our results suggested that the maximum accumulation (Accmax ) of CA and CS was mainly conferred to stimuli associated with oxidative stress such as UV-light exposure (Accmax , 6.2â
mg L-1 ) polyethylene glycol (Accmax , 6.5â
mg L-1 ) NaCl (Accmax , 5.9â
mg L-1 ) which simulated drought and saline stress, respectively. Nevertheless the bacterial elicitor pyocyanin was also effective to increase the production of both diterpenes (Accmax , 6.4â
mg L-1 ). Outstandingly, the incorporation of upstream biosynthetic precursors such as geranylgeraniol and miltiradiene/abietatriene, generated the best results with Accmax of 8.6 and 16.7â
mg L-1 , respectively. Optimized batch cultures containing 100â
mg L-1 geranylgeraniol, 50â
mg L-1 miltiradiene/abietatriene (95 5 %) and 5â
g L-1 polyethylene glycol treated with 6â
min UV light pulse during 30â
days resulted in Accmax of 26.7â
mg L-1 for CA and 17.3â
mg L-1 for CS on daysâ
18-24. This strategy allowed to increase seven folds the amounts of CA and CS in comparison with batch cultures without elicitation (Accmax , 4.3â
mg L-1 ).
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Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Plantas Medicinais
/
Lamiaceae
/
Diterpenos
Idioma:
En
Revista:
Chem Biodivers
Ano de publicação:
2023
Tipo de documento:
Article