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Omega-3 fatty acid and menaquinone-7 combination are helpful for aortic calcification prevention, reducing osteoclast area of bone and Fox0 expression of muscle in uremic rats.
Lee, Su Mi; Jeong, Eu Gene; Jeong, Yu In; Rha, Seo Hee; Kim, Seong Eun; An, Won Suk.
Afiliação
  • Lee SM; Department of Internal Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
  • Jeong EG; Department of Internal Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
  • Jeong YI; Department of Internal Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
  • Rha SH; Department of Pathology, College of Medicine, Dong-A University, Busan, Republic of Korea.
  • Kim SE; Department of Internal Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
  • An WS; Department of Internal Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea.
Ren Fail ; 44(1): 1873-1885, 2022 Dec.
Article em En | MEDLINE | ID: mdl-36632744
BACKGROUND: Osteopenia, sarcopenia, and vascular calcification (VC) are prevalent in patients with chronic kidney disease and often coexist. In the absence of proven therapies, it is necessary to develop therapeutic or preventive nutrients supplementation for osteopenia, sarcopenia, and VC. The present study investigated the effect of omega-3 fatty acid (FA) and menaquinone-7 (MK-7) on osteopenia, sarcopenia, and VC in adenine and low-protein diet-induced uremic rats. METHODS: Thirty-two male Sprague-Dawley rats were fed diets containing 0.75% adenine and 2.5% protein for three weeks. Rats were randomly divided into four groups that were fed diets containing 2.5% protein for four weeks: adenine control (0.9% saline), omega-3 FA (300 mg/kg/day), MK-7 (50 µg/kg/day), and omega-3 FA/MK-7. Von Kossa staining for aortic calcification assessment was performed. Osteoclast surface/bone surface ratio (OcS/BS) of bone and muscle fiber were analyzed using hematoxylin and eosin staining. Osteoprotegerin (OPG) immunohistochemical staining was done in the aorta and bone. Molecules related with sarcopenia were analyzed using western blotting. RESULTS: Compared to the normal control, OcS/BS and aortic calcification, and OPG staining in the aorta and bone were significantly increased in the adenine controls. OPG staining and aortic calcification progressed the least in the group supplemented with both omega-3 FA/MK-7. In the adenine controls, the regular arrangement of muscle fiber was severely disrupted, and inflammatory cell infiltration was more prominent. These findings were reduced after combined supplementation with omega-3 FA/MK-7. Furthermore, decreased mammalian target of rapamycin and increased Forkhead box protein 1 expression was significantly restored by combined supplementation. CONCLUSIONS: Combined nutrients supplementation with omega-3 FA and MK-7 may be helpful for aortic VC prevention, reducing osteoclast activation and improving sarcopenia-related molecules in adenine and low-protein diet induced uremic rats.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoclastos / Doenças da Aorta / Uremia / Doenças Ósseas Metabólicas / Ácidos Graxos Ômega-3 / Vitamina K 2 / Sarcopenia / Calcificação Vascular Tipo de estudo: Etiology_studies Idioma: En Revista: Ren Fail Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteoclastos / Doenças da Aorta / Uremia / Doenças Ósseas Metabólicas / Ácidos Graxos Ômega-3 / Vitamina K 2 / Sarcopenia / Calcificação Vascular Tipo de estudo: Etiology_studies Idioma: En Revista: Ren Fail Ano de publicação: 2022 Tipo de documento: Article