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[Anti-inflammatory mechanism of Balanophora involucrata: a network pharmacology and molecular docking-based analysis and verification in lipopolysaccharide-induced RAW264.7 cells].
Zhang, Y; Tu, X; Zhang, Y; Wen, D; Zhao, F; Yuan, L; Li, W.
Afiliação
  • Zhang Y; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases (Hubei Minzu University), Enshi 445000, China.
  • Tu X; School of Medicine, Hubei Minzu University, Enshi 445000, China.
  • Zhang Y; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases (Hubei Minzu University), Enshi 445000, China.
  • Wen D; Chinese Medicinal Materials Products Quality Supervision and Inspection Wenter in Wuling Mountainous Area, Enshi 445000, China.
  • Zhao F; School of Medicine, Hubei Minzu University, Enshi 445000, China.
  • Yuan L; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases (Hubei Minzu University), Enshi 445000, China.
  • Li W; Chinese Medicinal Materials Products Quality Supervision and Inspection Wenter in Wuling Mountainous Area, Enshi 445000, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 43(3): 383-392, 2023 Mar 20.
Article em Zh | MEDLINE | ID: mdl-37087582
ABSTRACT

OBJECTIVE:

To investigate the main chemical constituents of Balanophora involucrata and the mechanism of its antiinflammatory effect based on network pharmacology and molecular docking technology.

METHODS:

Literature reports, Materia Medica, GeneCards and other databases were searched for anti-inflammatory compounds and their targets. String database and Cytoscape 3.7.2 software were used to obtain the protein-protein interaction (PPI) network and the drug-active ingredienttargets network and for GO and KEGG enrichment analyses. Molecular docking was performed using Auto Dock Tools 1.5.6. In an inflammatory RAW264.7 cell model induced by lipopolysaccharide (LPS), the effect of 25, 50, 100, 200 µg/mL Balanophora involucrata extract was tested on the production of inflammatory cytokines and phosphorylation level of PI3K and Akt using ELISA and Western blotting.

RESULTS:

A total of 318 common targets of drugs and diseases were identified, and the core targets were Src, HSP90AA1 and PIK3CA, involving cancer, PI3K/Akt, MAPK and other signaling pathways as shown by KEGG analysis. Molecular docking showed that both the main active constituents of Balanophora involucrata could spontaneously bind to the core targets. In the inflammatory cell model, treatment with Balanophora involucrata extract significantly inhibited the production of IL-1ß at the concentrations of 100 and 200 µg/mL, reduced IL-6 and TNF-α expressions at the concentrations of 50, 100, and 200 µg/mL, and lowered phosphorylation levels of PI3K and Akt proteins at the concentrations of 25, 50, 100, and 200 µg/mL (all P < 0.05).

CONCLUSIONS:

The anti-inflammatory mechanism of Balanophora involucrata involves multiple targets and multiple pathways, and its effect is mediated possibly by reducing IL-1ß, IL-6 and TNF-α production and inhibiting phosphorylation levels of PI3K and Akt proteins to suppress the activation of the PI3K/Akt signaling pathway.
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Texto completo: 1 Base de dados: MEDLINE Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Medicinas Complementares: Homeopatia Assunto principal: Medicamentos de Ervas Chinesas / Farmacologia em Rede Tipo de estudo: Prognostic_studies Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Medicinas Complementares: Homeopatia Assunto principal: Medicamentos de Ervas Chinesas / Farmacologia em Rede Tipo de estudo: Prognostic_studies Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China