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Evening primrose oil enriched with gamma linolenic acid and D/L-alpha tocopherol acetate attenuated carbon tetrachloride-induced hepatic injury model in male rats via TNF-α, IL-1ß, and IL-6 pathway.
Gad El-Hak, Heba Nageh; Kishk, Safaa M; Abdelrazek, Heba M A.
Afiliação
  • Gad El-Hak HN; Zoology Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt.
  • Kishk SM; Department of Medicinal Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
  • Abdelrazek HMA; Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Toxicol Mech Methods ; 34(5): 469-483, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38166523
ABSTRACT
The modulatory role of primrose oil (PO) supplementation enriched with γ-linolenic acid and D/L-alpha tocopherol acetate against a carbon tetrachloride (CCl4)-induced liver damage model was assessed in this study. Twenty male Albino rats were divided into four groups. The control group received corn oil orally. The PO group received 10 mg/kg P O orally. The CCl4 group received 2 mL/kg CCl4 orally and PO/CCl4 group; received PO and 2 mL/kg CCl4 orally. The relative liver weight was recorded. Serum liver enzymes, hepatic malondialdehyde (MDA), hepatic reduced glutathione (GSH) and the expression of hepatic tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1ß), and interleukin 6 (IL-6) were assessed. The binding affinities of γ-linolenic acid and D/L-alpha tocopherol constituents with IL-1ß, IL-6 and TNF-α were investigated using molecular docking simulations. Histopathological and electron microscopic examinations of the liver were performed. The results indicated that CCl4 elevated serum liver enzyme and hepatic MDA levels, whereas GSH levels were diminished. The upregulation of IL-1ß, IL-6, and TNF-α gene expressions were induced by CCl4 treatment. The PO/CCl4-treated group showed amelioration of hepatic injury biomarkers and oxidative stress. Restoration of histopathological and ultrastructural alterations while downregulations the gene expressions of TNF-α, IL1-ß and IL-6 were observed. In conclusion, evening primrose oil enriched with γ-linolenic acid and D/L-alpha tocopherol acetate elicited a potential amelioration of CCl4-induced hepatic toxicity.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óleos de Plantas / Oenothera biennis / Ácido gama-Linolênico / Doença Hepática Induzida por Substâncias e Drogas / Fígado Tipo de estudo: Prognostic_studies Idioma: En Revista: Toxicol Mech Methods Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Egito

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óleos de Plantas / Oenothera biennis / Ácido gama-Linolênico / Doença Hepática Induzida por Substâncias e Drogas / Fígado Tipo de estudo: Prognostic_studies Idioma: En Revista: Toxicol Mech Methods Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Egito