Propofol Modulates Gallbladder Cancer Progression via miR-4430/SLC19A3 Axis.
Altern Ther Health Med
; 30(10): 336-341, 2024 Oct.
Article
em En
| MEDLINE
| ID: mdl-38330587
ABSTRACT
Background/Aim:
Propofol is a common anesthetic used during surgery. MircoRNA (miRNA), especially miR-4430, is currently a research hotspot in GBC. This study investigates whether propofol regulates GBC through the miR-4430/SLC19A3 axis. Materials andmethods:
NOZ and SGC996 cells, human gallbladder cancer cell lines, were obtained from BLUEFBIO (Shanghai, China). The cells were treated with propofol, and the experimental setup included a control group. qRT-PCR was used to measure the content of miR-4430 and solute carrier family 19 member 3 (SLC19A3). The cell viability, migration, and apoptosis were analyzed by colony formation assay, wound healing assay, and flow cytometry, respectively. Western blot tested the levels of SLC91A3, cyclin D-dependent kinases 6 (CDK6), and Bax proteins. Mechanically, dual-luciferase reporter assay notarized the link of miR-4430 with SLC19A3.Results:
Propofol significantly repressed the proliferation of GBC cells, as indicated by a pronounced reduction in colony formation in both NOZ and SGC996 cells (P = .025, 95% CI1.2, 2.4). The inhibitory effect of propofol was reversed by the enhanced expression of miR-4430 in GBC cells. Furthermore, SLC19A3 was identified as a direct target of miR-4430. Silencing SLC19A3 reversed the altered cell behavior observed in propofol-treated GBC cells (P = .019, 95% CI1.6, 3.5). Additionally, overexpression of SLC19A3 significantly attenuated both cell proliferation and migration influenced by miR-4430 in propofol-treated GBC cells (P = .034, 95% CI1.4, 2.8).Conclusion:
Propofol changed the cell behaviors of GBC by modulating the miR-4430/SLC19A3 axis.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Propofol
/
MicroRNAs
/
Proliferação de Células
/
Neoplasias da Vesícula Biliar
Idioma:
En
Revista:
Altern Ther Health Med
Ano de publicação:
2024
Tipo de documento:
Article