Isolation, purification, and structural characterization of polysaccharides from Codonopsis pilosula and its therapeutic effects on non-alcoholic fatty liver disease in vitro and in vivo.
Int J Biol Macromol
; 265(Pt 2): 130988, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38518942
ABSTRACT
Codonopsis pilosula is a famous edible and medicinal plants, in which polysaccharides are recognized as one of the important active ingredients. A neutral polysaccharide (CPP-1) was purified from C. pilosula. The structure was characterized by HPSEC-MALLS-RID, UV, FT-IR, GC-MS, methylation analysis, and NMR. The results showed that CPP-1 was a homogeneous pure polysaccharide, mainly containing fructose and glucose, and a small amount of arabinose. Methylation analysis showed that CPP-1 composed of â1)-Fruf-(2â, Fruf-(1â and Glcp-(1â residues. Combined the NMR results the structure of CPP-1 was confirmed as α-D-Glcp-(1 â [2)-ß-D-Fruf-(1 â 2)-ß-D-Fruf-(1]26 â 2)-ß-D-Fruf with the molecular weight of 4.890 × 103 Da. The model of AML12 hepatocyte fat damage was established in vitro. The results showed that CPP-1 could increase the activity of SOD and CAT antioxidant enzymes and reduce the content of MDA, thus protecting cells from oxidative damage. Subsequently, the liver protective effect of CPP-1 was studied in the mouse model of nonalcoholic fatty liver disease (NAFLD) induced by the high-fat diet. The results showed that CPP-1 significantly reduced the body weight, liver index, and body fat index of NAFLD mice, and significantly improved liver function. Therefore, CPP-1 should be a potential candidate for the treatment of NAFLD.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Codonopsis
/
Hepatopatia Gordurosa não Alcoólica
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2024
Tipo de documento:
Article