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Sarcandra glabra (Thunb.) Nakai alleviates DSS-induced ulcerative colitis by promoting restitution, restoring barrier function, and modulating IL-17/Notch1/FoxP3 pathway in intestinal cells.
Zhang, Shuling; Li, Yanwu; Chen, Gang; Wang, Xu; Wu, Bin.
Afiliação
  • Zhang S; Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, 110004, China. Electronic address: slzhang@cmu.edu.cn.
  • Li Y; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China. Electronic address: liyanwu@gzucm.edu.cn.
  • Chen G; School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang, 110016,
  • Wang X; Department of Gastroenterological Endoscopy, The First Hospital of China Medical University, Shenyang, 110001, China. Electronic address: wangxu@cmu.edu.cn.
  • Wu B; Department of Hematology, Shengjing Hospital of China Medical University, Shenyang, 110004, China. Electronic address: vini_wb@163.com.
J Ethnopharmacol ; 328: 118131, 2024 Jun 28.
Article em En | MEDLINE | ID: mdl-38565408
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Sarcandra glabra is officially named Zhong Jie Feng as a traditional medicine. In the nationality of Yao and Zhuang, it has been used to treat digestive diseases like stomachache and dysentery. Similarly, in Dai nationality, it has been used to treat intestinal diseases like gastric ulcers. However, the effect and mechanism of S. glabra on experimental ulcerative colitis (UC) are known. AIM OF STUDY The main objective of this study was to investigate the effect and mechanism of S. glabra on experimental UC. MATERIALS AND

METHODS:

The chemical components in the water extract of S. glabra (ZJF) were analyzed by UPLC-MS/MS method. The HCoEpiC cell line was used to assess the promotive effect on intestinal proliferation and restitution. RAW264.7 cells were used to assess the in vitro anti-inflammatory effect of ZJF. The 3% DSS-induced colitis model was used to evaluate the in vivo effect of ZJF (4.5 g/kg and 9.0 g/kg). Mesalazine (0.5 g/kg) was used as the positive drug. ELISA, RT-qPCR, Western blot, and multiplex immunohistochemical experiments were used to test gene levels in the colon tissue. The H&E staining method was used to monitor the pathological changes of colon tissue. TUNEL assay kit was used to detect apoptosis of epithelial colonic cells.

RESULTS:

ZJF could alleviate the DSS-caused colitis in colon tissues, showing a comparative effect to that of the positive drug mesalazine. Mechanism study indicated that ZJF could promote normal colonic HCoEpiC cell proliferation and restitution, inhibit overexpression of pro-inflammatory cytokines, restore the M1/M2 ratio, decrease epithelial colonic cell apoptosis, rescue tight junction protein levels, and modulate IL-17/Notch1/FoxP3 pathway to treat experimental UC.

CONCLUSION:

Our results indicated that S. glabra can promote intestinal cell restitution, balance immune response, and modulate IL-17/Notch1/FoxP3 pathway to treat experimental UC.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Colite Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Colite Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2024 Tipo de documento: Article