An anti-A1 lectin in the seeds of Amphicarpaea bracteata.
Biochim Biophys Acta
; 532(2): 225-31, 1978 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-623780
ABSTRACT
An anti-A1 lectin has been isolated from the extract of Amphicarpaea bracteata seeds by affinity chromatography on Epoxy-activated Sepharose 6B coupled to N-acetyl-D-galactosamine. The yield of the purified lectin was 86 microgram/g of seeds. The purified lectin shows one main band on electrophoresis in sodium dodecyl sulfate-polyacrylamide. The amino acid and neutral sugar composition indicate that this lectin is an acidic glycoprotein with a neutral sugar content of approx. 2%. The composition of the lectin is different from that of the Dolichos biflorus lectin but the two lectins have some common characteristics. The most powerful inhibitors of the agglutination of A1 red blood cells by the A. bracteata lectin is N-acetyl-D-galactosamine. Much weaker inhibitors of the agglutination are alpha-lactose, D-fucose, and five other sugars.
Buscar no Google
Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Plantas Medicinais
/
Sistema ABO de Grupos Sanguíneos
/
Lectinas
/
Fabaceae
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1978
Tipo de documento:
Article