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Growth and maturation of small hepatocytes isolated from adult rat liver.
Mitaka, T; Kojima, T; Mizuguchi, T; Mochizuki, Y.
Afiliação
  • Mitaka T; Department of Pathology, Sapporo Medical University School of Medicine, Japan.
Biochem Biophys Res Commun ; 214(2): 310-7, 1995 Sep 14.
Article em En | MEDLINE | ID: mdl-7677736
Small hepatocytes existed in the supernatant following low-speed centrifugation of the cell suspension after collagenase liver perfusion. The cells proliferated for more than 2 months and formed colonies in the Dulbecco's modified Eagle's medium supplemented with 10 mM nicotinamide, 10% fetal bovine serum, 1 mM ascorbic 2-phosphate, and 10 ng/ml epidermal growth factor. One small cell finally proliferated to several hundred cells. In addition, some cells in the colonies were shown to differentiate into mature hepatocytes that had a large cytoplasm and sometimes two nuclei. The secretion of albumin in the medium by the hepatocytes increased with time in culture, and the cells possessed connexin 32 in their cell membrane and many peroxisomes. Thus, the small hepatocytes may be "committed progenitor cells" which can further differentiate into mature hepatocytes.
Assuntos
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Base de dados: MEDLINE Assunto principal: Fígado Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Japão
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Base de dados: MEDLINE Assunto principal: Fígado Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Japão