Cardiac sarcoplasmic reticulum Ca(2+)-ATPase expression in streptozotocin-induced diabetic rat heart.
Biochem Biophys Res Commun
; 203(1): 113-20, 1994 Aug 30.
Article
em En
| MEDLINE
| ID: mdl-8074644
ABSTRACT
Chronic diabetes due to streptozotocin administration has been shown to induce heart dysfunction characterized by prolonged relaxation time as well as decreased Ca2+ transport and Ca(2+)-ATPase (SERCA2) activities of the cardiac sarcoplasmic reticulum (SR). Rats made diabetic with 65 mg/kg of streptozotocin for 3 and 5 weeks exhibited decreased SR Ca(2+)-pump activities; these were normalized upon treatment with insulin. Northern blot and slot blot analyses did not show statistically significant reduction in the relative level of SERCA2 mRNA expression in diabetic or insulin treated rats. Quantitation of SERCA2 protein by Western blot did not reveal any change in diabetic and insulin treated animals. These results suggest that the defect in SR Ca(2+)-pump may not be due to changes at the transcriptional or translational levels in the diabetic heart.
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Base de dados:
MEDLINE
Assunto principal:
Retículo Sarcoplasmático
/
Expressão Gênica
/
ATPases Transportadoras de Cálcio
/
Diabetes Mellitus Experimental
/
Miocárdio
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Canadá