Pollen of a male-sterile mutant of Arabidopsis thaliana isolated from a T-DNA insertion pool is not effectively released from the anther locule.
Plant Cell Physiol
; 37(5): 580-5, 1996 Jul.
Article
em En
| MEDLINE
| ID: mdl-8819307
ABSTRACT
We have isolated a male-sterile mutant from a pool of T-DNA insertional lines of Arabidopsis thaliana generated by an in planta transformation procedure [Chang et al. (1994) Plant J. 5 551]. Pollen in this mutant is not effectively released from anther locules after cleavage of the stomium. Most mutant pollen grains are round, in contrast to the tricolpate wild-type pollen, and some pollen grains show an abnormal surface structure. Manually released mutant pollen grains are not fertile and show defects in pollen tube germination in vitro. Genetic analysis disclosed that this lesion is due to a single recessive nuclear mutation located on chromosome 3 closely linked to the gll locus. The mutation locus is tightly linked to the inserted T-DNA.
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Base de dados:
MEDLINE
Assunto principal:
Pólen
/
Arabidopsis
/
DNA de Plantas
Idioma:
En
Revista:
Plant Cell Physiol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Coréia do Sul