A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens.
Insect Mol Biol
; 7(2): 107-20, 1998 May.
Article
em En
| MEDLINE
| ID: mdl-9535157
ABSTRACT
An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.
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Base de dados:
MEDLINE
Assunto principal:
Acetilcolinesterase
/
Culex
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Insect Mol Biol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Reino Unido