Involvement of tyrosine phosphorylation of p185(c-erbB2/neu) in tumorigenicity induced by X-rays and the neu oncogene in human breast epithelial cells.
Mol Carcinog
; 21(4): 225-33, 1998 Apr.
Article
em En
| MEDLINE
| ID: mdl-9585252
ABSTRACT
Ionizing radiation is the exogenous agent best proven to induce breast cancer. c-erbB2/neu amplification and overexpression are known to occur in breast cancer and are correlated with aggressive tumor growth and poor prognosis. We have developed simian virus 40-immortalized cell lines from normal human breast epithelial cells (HBECs) with luminal and stem-cell characteristics. In this study, we examined whether x-rays and a mutated neu oncogene are capable of inducing tumorigenicity in these cells. The results indicated that x-rays were effective in converting immortal non-tumorigenic HBECs to weakly tumorigenic cells that then could be transformed to highly tumorigenic cells by the neu oncogene. The in vitro growth of these tumorigenic cells was significantly faster than that of the parental non-tumorigenic cells in growth factor- and hormone-supplemented or -depleted media. The neu oncogene, however, had no tumorigenic effect on immortal non-tumorigenic cells. The expression of p185(c-erb82/neu) was elevated in neu-transduced immortal or weakly tumorigenic cell lines. However, only in the latter was p185(c-erbB2/neu) found to be phosphorylated at tyrosine residues. Thus, x-rays appear to induce a genetic alteration that confers weak tumorigenicity on immortal HBECs and interacts with p185(c-erbB2/neu) directly or indirectly to give rise to fast-growing tumors.
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Base de dados:
MEDLINE
Assunto principal:
Mama
/
Transformação Celular Neoplásica
/
Processamento de Proteína Pós-Traducional
/
Receptor ErbB-2
/
Genes erbB-2
/
Neoplasias Mamárias Experimentais
/
Neoplasias Induzidas por Radiação
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Idioma:
En
Revista:
Mol Carcinog
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos