Redox Regulating Protein APE1/Ref-1 Expression is Increased in Abdominal Aortic Coarctation-induced Hypertension Rats
Journal of the Korean Society of Hypertension
; : 126-135, 2012.
Article
em Ko
| WPRIM
| ID: wpr-20164
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Aim of study is designed to investigate whether apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE1/Ref-1) expression is changed in abdominal aortic coarctation models. METHODS: Male Sprague-Dawley rats were randomly assigned with abdominal aortic coarctation, repaired group, sham, and control groups. Endothelial function was assessed with endothelium-dependent relaxations. Detection of superoxide anion and lipid peroxidation was performed by lucigenin chemiluminescence and thiobarbituric acid-reactive substances assay. APE1/Ref-1 expression was measured with Western blot and immunohistochemistry. RESULTS: In anesthetized condition, the abdominal aortic coarctation rats showed hypertension as systolic/diastolic arterial pressure of 171/114 mm Hg, compared with 114/94 mm Hg of control. Endothelium-dependent relaxations were significantly impaired in the aortic coarctation which was recovered in 1 week after coarctation repair. Superoxide production and lipid peroxidation were elevated in aortic coarctation rats. In immunohistochemistry, APE1/Ref-1 expressions were increased at aorta and kidney in aortic coarctation rats. Increased APE1/Ref-1 expression in aorta was recovered by repair of coarctation. CONCLUSIONS: Taken together, it suggests that APE1/Ref-1 expression was increased in aortic coarctation-induced hypertensive rats, suggesting a biomarker for hypertension. Impaired endothelium dependent relaxation in the aortic coarctation can be modulated by repair of coarctation or the modulation of blood pressure.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Métodos Terapêuticos e Terapias MTCI:
Terapias_mente_y_cuerpo
/
Relajacion
Assunto principal:
Aorta
/
Coartação Aórtica
/
Oxirredução
/
Relaxamento
/
Acridinas
/
Pressão Sanguínea
/
Imuno-Histoquímica
/
Peroxidação de Lipídeos
/
Salicilamidas
/
Western Blotting
Tipo de estudo:
Prognostic_studies
Idioma:
Ko
Revista:
Journal of the Korean Society of Hypertension
Ano de publicação:
2012
Tipo de documento:
Article