The effect of Siwu Tang on EPO and G-CSF gene expression in bone marrow of irradiated blood deficiency mice / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1173-1176, 2005.
Article
em Zh
| WPRIM
| ID: wpr-239723
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.</p><p><b>METHOD</b>The animal model of blood deficiency were established in the mice by using 3.5 Gy60Co gamma-ray. Peripheral blood cells were analyzed and CFU-GM, BFU-E, CFU-E and CFU-mix were counted in bone marrow colony cultured. Both Epo and G-CSF gene expressions in bone marrow were measured with RT-PCR.</p><p><b>RESULT</b>Siwu Tang significantly increased the number of peripheral blood cells and the amount of CFU-GM, BFU-E, CFU-E and CFU-mix in bone marrow and enhanced Epo and G-CSF gene expression in bone marrow in the mice with blood deficiency.</p><p><b>CONCLUSION</b>The promotion of Epo and G-CSF gene expressions in bone marrow may be one of the mechanisms underlying the blood enriching function of Siwu Tang decoction.</p>
Texto completo:
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Base de dados:
WPRIM
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Plantas_medicinales
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
Medula Óssea
/
RNA Mensageiro
/
Células da Medula Óssea
/
Medicamentos de Ervas Chinesas
/
Células Precursoras Eritroides
/
Contagem de Células
/
Química
/
Fator Estimulador de Colônias de Granulócitos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Article