Mongolian medicine cha gan beng ga regulated activity of biomarker PGC-1α / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3371-3375, 2014.
Article
em Zh
| WPRIM
| ID: wpr-244561
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulation of Cha Gan Beng Ga on the activity of biomarker PGC-1α in vivo and in vitro, and lay the foundation for studying the efficacy result of Cha Gan Beng Ga on xenograft tumor model and extracting active constituents.</p><p><b>METHOD</b>(1) The coarse powder of Cha Gan Beng Ga was extracted with 70% ethanol solution through heating and refluxing, and finally was used to freeze dry powder. (2) 50 mg x kg(-1) of freeze-dried power was orally administrated to KM and C57BL/6J mice once daily, lasting for 5 consecutive days; different concentrations of extracted materials was given to non-small cell lung cells A549. (3) The expression level of PGC-1α mRNA was quantitatively determined in lung tissue of mice and non-small cell lung cells A549.</p><p><b>RESULT</b>The expression levels of PGC-1α in lung tissue of different mice strains had an increasing tendency. Furthermore, the expression levels of PGC-1α in non-small cell lung cells A549 also had an increasing tendency, showing dose and time-dependent relationships.</p><p><b>CONCLUSION</b>Mongolian Medicine Cha Gan Beng Ga could induce the over-expression of PGC-1α mRNA in lung tissue of mice and in non-small cell lung cells A549. The present results will lay foundation for studying the efficacy result of antitumor and active constitutes in future.</p>
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Base de dados:
WPRIM
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Patologia
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Farmacologia
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Fatores de Tempo
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Fatores de Transcrição
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Extratos Vegetais
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Biomarcadores Tumorais
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Regulação Neoplásica da Expressão Gênica
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Química
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Carcinoma Pulmonar de Células não Pequenas
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article