In vitro effect of total flavones of Fructus Chorspondiatis on expression of collagen type I and type III mRNA and protein of cultured rat cardiac fibroblasts / 药学学报
Acta Pharmaceutica Sinica
; (12): 136-141, 2014.
Article
em Zh
| WPRIM
| ID: wpr-245089
Biblioteca responsável:
WPRO
ABSTRACT
This study aims to investigate the effect of total flavones of Fructus Chorspondiatis (TFFC) on the mRNA and protein expression of collagen type I and III of rat cardiac fibroblasts (CFs) induced by angiotensin II (Ang II), and explore its anti-myocardial fibrosis molecular mechanism. Neonatal rat CFs were prepared from Sprague-Dawley rats (1-3 d after birth). The expression of collagen type I and III mRNA and protein were measured by RT-PCR and Western blotting, respectively. The study showed that stimulation of neonatal rat CFs with 100 nmol.L-1 of Ang II for 72 h resulted in a significant increase of the expression of collagen type I and III mRNA and protein. The changes on the expression level were blocked by TFFC. The results demonstrated that TFFC can inhibit myocardial fibrosis induced by Ang II in rats, which is probably associated with the collagen type I and III mRNA and protein levels up-regulated by Ang II, and TFFC was shown to decrease the expression levels of collagen type I and III mRNA and protein.
Texto completo:
1
Base de dados:
WPRIM
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Farmacologia
/
Plantas Medicinais
/
RNA Mensageiro
/
Angiotensina II
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Medicamentos de Ervas Chinesas
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Células Cultivadas
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Química
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Ratos Sprague-Dawley
/
Anacardiaceae
/
Biologia Celular
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2014
Tipo de documento:
Article