DNA extraction and sex determination for human teeth dated 3000 years ago unearthed in Xi'an / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 619-621, 2013.
Article
em Zh
| WPRIM
| ID: wpr-254549
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi'an and determine the genders for the individuals.</p><p><b>METHODS</b>Thirty five ancient human teeth were studied. A 'Reverse-root-canal' technique and a Chelex-100 solution were used to extract the DNA. Specific primers for Amelogenin gene were designed for PCR amplification.</p><p><b>RESULTS</b>Genomic DNA was successfully extracted from 30 samples, for which 8 were determined to be males and 22 were females.</p><p><b>CONCLUSION</b>The 'Reverse-root-canal' technique may be used for extracting DNA from ancient human teeth. Genetics method can supplement physical anthropology for determination of sex for ancient samples.</p>
Texto completo:
1
Base de dados:
WPRIM
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Paleodontologia
/
Análise para Determinação do Sexo
/
Dente
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DNA
/
Química
/
China
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Reação em Cadeia da Polimerase
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História Antiga
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Amelogenina
/
Genética
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2013
Tipo de documento:
Article