Determination of ligustilide in volatile oil from rhizome of ligusticum chuanxiong by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC.</p><p><b>METHOD</b>ODS2 column (4.6 mm x 200 mm, 5 microm) was used and nitrendipine was used as internal standard. The mobil phase consisted methanol, acetontrile and water (33:21:46). The ligustilide was at 275 nm.</p><p><b>RESULT</b>The linear range was 2.92-29.2 mg x L(-1) for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%.</p><p><b>CONCLUSION</b>This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.</p>