Lycopene attenuates angiotensin II induced oxidative stress in H9c2 cells / 中华心血管病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Lycopene (Lyc) on Ang II induced oxidative stress in H9c2 cell line derived from rat cardiac tissue,and to explore related mechanisms.</p><p><b>METHODS</b>H9c2 cells were divided into 6 groups: control group, Ang II group (1 µmol/L), Ang II (1 µmol/L) + low dose Lyc (3.125 nmol/L) group, Ang II (1 µmol/L) + moderate dose Lyc (6.25 nmol/L) group and Ang II (1 µmol/L) + high dose Lyc (12.5 nmol/L) group and Lyc group (12.5 nnmol/L). Cell growth was determined by CCK8 assay, ROS generation was detected using a Microplate reader and Fluorescence microscopy, the expression of NOX2 was determined by Western blot, mRNA expression of p47(phox), SOD1 and SOD2 were determined by Real Time-PCR, MDA was detected by ELISA kit.</p><p><b>RESULTS</b>Compared to control group,cell survival was significantly reduced and ROS generation was significantly increased post Ang II stimulation,cotreatment with Lyc significantly improved cell survival and reduced ROS generation in a dose-dependent manner (all P < 0.01). mRNA expression of SOD1 and SOD2 was significantly downregulated while MDA concentration was significantly increased in Ang II treated cells, which could be significantly reversed by cotreatment with Lyc in a dose dependent manner (all P < 0.01). Protein expression of NOX2 and mRNA expression of p47(phox) were significantly upregulated post Ang II and which could be significantly downregulated by cotreatment with Lyc in a dose-dependent manner (all P < 0.01).</p><p><b>CONCLUSION</b>Lyc could attenuate Ang II induced oxidative stress and this effect is linked with its capacity of reducing ROS generation and enhancing cellular ROS scavenging ability in H9c2 cells.</p>