DNA barcoding identification between arisaematis rhizoma and its adulterants based on ITS2 sequences / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2176-2179, 2014.
Article
em Zh
| WPRIM
| ID: wpr-330326
Biblioteca responsável:
WPRO
ABSTRACT
Fifty-eight samples belonging to 7 species of Arisaematis Rhizoma and its adulterants were collected. The ITS2 locus was employed as a DNA barcode and amplified, sequenced and assembled for all of the collected samples. Then, ITS2 sequences have been annotated using HMM-based method. The intra- and inter-specific variations were calculated and NJ tree was constructed using MEGA 6.0 software. The results showed that inter-specific K2P distances were significantly larger than intra-specific distances for all of the three origin species of Arisaematis Rhizoma. Furthermore, three origin species, Arisaema amurense, A. erubescens and A. heterophyllum, can be respectively formed to be a single branch with high bootstrap values. It is concluded that ITS2 can be used to correctly identify Arisaematis Rhizoma from its adulterants and the application of ITS2 in the identification of traditional Chinese medicine has an important prospective.
Texto completo:
1
Base de dados:
WPRIM
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Filogenia
/
Controle de Qualidade
/
Medicamentos de Ervas Chinesas
/
Dados de Sequência Molecular
/
Química
/
Contaminação de Medicamentos
/
Classificação
/
DNA de Plantas
/
DNA Espaçador Ribossômico
/
Rizoma
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article