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Shenshuai Yingyang capsule ameliorates muscle atrophy in rats with chronic renal failure: role of Wnt7a-Akt/mTOR signal pathway / 南方医科大学学报
Article em Zh | WPRIM | ID: wpr-333662
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Shenshuai Yingyang Capsule (SSYYJN) in ameliorating muscle atrophy in rats with chronic renal failure (CRF) and explore the role of Wnt7a-Akt/mTOR signal pathway in mediating this effect.</p><p><b>METHODS</b>Male rats were randomly assigned to 5/6 nephrectomy group and sham-operated group, and the former group was further randomly divided into CRF model group, KA group, and SSYYJN group. The size of anterior tibia muscle was examined microscopically with HE staining. Protein synthesis in the soleus muscle was investigated by (14)C-phenylalanine experiment, and the expression of Wnt7a, frizzled-7, phospho-Akt, phospho-mTOR and GAPDH were detected with Western blotting.</p><p><b>RESULTS</b>The body weight, the wet and dry weight, cross-sectional area, and muscle protein synthesis of the anterior tibia muscles, and expressions of the proteins in the Wnt7a/Akt signaling pathway all increased significantly in SSYYJN and KA groups as compared with those in the model group.</p><p><b>CONCLUSION</b>SSYYJN can effectively improve muscle atrophy in the rat model of CRF possibly by reversing the reduction in the expressions of Wnt7a/Akt signaling pathway proteins in the skeletal muscles.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Assunto principal: Farmacologia / Cápsulas / Medicamentos de Ervas Chinesas / Atrofia Muscular / Transdução de Sinais / Proteínas Proto-Oncogênicas / Músculo Esquelético / Tratamento Farmacológico / Proteínas Wnt / Serina-Treonina Quinases TOR Tipo de estudo: Prognostic_studies Idioma: Zh Revista: Journal of Southern Medical University Ano de publicação: 2015 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Medicinas Tradicionais: Medicinas_tradicionales_de_asia / Medicina_china Assunto principal: Farmacologia / Cápsulas / Medicamentos de Ervas Chinesas / Atrofia Muscular / Transdução de Sinais / Proteínas Proto-Oncogênicas / Músculo Esquelético / Tratamento Farmacológico / Proteínas Wnt / Serina-Treonina Quinases TOR Tipo de estudo: Prognostic_studies Idioma: Zh Revista: Journal of Southern Medical University Ano de publicação: 2015 Tipo de documento: Article