Characterization of Echinostoma cinetorchis endoribonuclease, RNase H
The Korean Journal of Parasitology
; : 451-455, 2017.
Article
em En
| WPRIM
| ID: wpr-69358
Biblioteca responsável:
WPRO
ABSTRACT
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3′-O-P bond of RNA in a DNA-RNA duplex, producing 3′-hydroxyl and 5′-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Parasitos
/
Ribonucleases
/
Trematódeos
/
RNA
/
Oligonucleotídeos Antissenso
/
DNA Complementar
/
Ribonuclease H
/
Domínio Catalítico
/
Echinostoma
/
Endorribonucleases
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2017
Tipo de documento:
Article