1-Methoxycarbony-β-carboline from Picrasma quassioides exerts anti-angiogenic properties in HUVECs in vitro and zebrafish embryos in vivo / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 599-609, 2018.
Article
em En
| WPRIM
| ID: wpr-773581
Biblioteca responsável:
WPRO
ABSTRACT
Angiogenesis is a crucial process in the development of inflammatory diseases, including cancer, psoriasis and rheumatoid arthritis. Recently, several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model, and the results indicated that 1-methoxycarbony-β-carboline (MCC) could effectively inhibit blood vessel formation. In this study, we further confirmed that MCC can inhibit, in a concentration-dependent manner, the viability, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro, as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo. In the zebrafish xenograft assay, MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells. The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins, including ANG, EGF, bFGF, GRO, IGF-1, PLG and MMP-1. In addition, the expression of two key membrane receptor proteins in angiogenesis, TIE-2 and uPAR, were also down-regulated after MCC treatment. Taken together, these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Peixe-Zebra
/
Fator de Crescimento Insulin-Like I
/
Carbolinas
/
Extratos Vegetais
/
Movimento Celular
/
Química
/
Embriologia
/
Neovascularização Fisiológica
/
Inibidores da Angiogênese
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2018
Tipo de documento:
Article