Transcriptomic analysis in Anemone flaccida rhizomes reveals ancillary pathway for triterpene saponins biosynthesis and differential responsiveness to phytohormones / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 131-144, 2019.
Article
em En
| WPRIM
| ID: wpr-776900
Biblioteca responsável:
WPRO
ABSTRACT
Anemone flaccida Fr. Schmidt is a perennial medicinal herb that contains pentacyclic triterpenoid saponins as the major bioactive constituents. In China, the rhizomes are used as treatments for a variety of ailments including arthritis. However, yields of the saponins are low, and little is known about the plant's genetic background or phytohormonal responsiveness. Using one-quarter of the 454 pyrosequencing information from the Roche GS FLX Titanium platform, we performed a transcriptomic analysis to identify 157 genes putatively encoding 26 enzymes involved in the synthesis of the bioactive compounds. It was revealed that there are two biosynthetic pathways of triterpene saponins in A. flaccida. One pathway depends on β-amyrin synthase and is similar to that found in other plants. The second, subsidiary ("backburner") pathway is catalyzed by camelliol C synthase and yields β-amyrin as minor byproduct. Both pathways used cytochrome P450-dependent monooxygenases (CYPs) and family 1 uridine diphosphate glycosyltransferases (UGTs) to modify the triterpenoid backbone. The expression of CYPs and UGTs were quite different in roots treated with the phytohormones methyl jasmonate, salicylic acid and indole-3-acetic acid. This study provides the first large-scale transcriptional dataset for the biosynthetic pathways of triterpene saponins and their phytohormonal responsiveness in the genus Anemone.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Ácido Oleanólico
/
Farmacologia
/
Reguladores de Crescimento de Plantas
/
Proteínas de Plantas
/
Plantas Medicinais
/
Saponinas
/
Triterpenos
/
Glicosiltransferases
/
Regulação da Expressão Gênica de Plantas
/
Perfilação da Expressão Gênica
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2019
Tipo de documento:
Article