Influence of ultrafiltration membrane on ophiopogonins and homoisoflavonoids in Ophiopogon japonicus as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 121-141, 2017.
Article
em En
| WPRIM
| ID: wpr-812132
Biblioteca responsável:
WPRO
ABSTRACT
Ultrafiltration is one of the most fascinating technologies, which makes it possible to improve the quality of traditional medicines for application in the pharmaceutical industry. However, researchers have paid little attention to the effect of ultrafiltration membrane on traditional medicines chemical constituents. In this work, Ophiopogon japonicus (L.f) Ker-Gawl. was used as an example to illuminate the influence of ultrafiltration with different material and molecular weight cut-off (MWCO) membrane on natural chemical constituents as measured by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Our results indicated that ultrafiltration membrane significantly impacted homoisoflavonoids, especially homoisoflavonoids that were almost completely retained on the polyethersulfone (PES) membrane. We also found that the larger number of aglycone hydroxy and sugar moiety in steroid saponins, the higher the transmittance. Furthermore, the passage rate (%) of ophiogenin type saponins was higher than that of others. The possible adsorptive mechanisms were hydrogen bonding, hydrophobic interactions, and benzene ring interaction by π-π stacking. In conclusion, it is crucial to choose appropriate ultrafiltration membrane based on the characteristics of produce products for application of ultrafiltration technique.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Polímeros
/
Saponinas
/
Sulfonas
/
Medicamentos de Ervas Chinesas
/
Extratos Vegetais
/
Ultrafiltração
/
Estrutura Molecular
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Cromatografia Líquida
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2017
Tipo de documento:
Article