Fingerprint of different polarity extracts from superfine powder of Lonicera japonica and its correlation of antibacterial activity / 中草药

ABSTRACT

Objective: To establish the fingerprint of Lonicera japonica superfine powder with different polarity and study its correlation of antibacterial activity. Methods: Six different polarity extracts were obtained using the solvent system extraction and then were detected by HPLC with gradient elution. Pharmacological activities were detected by antibiotic indexes in vitro. The correlation between the pharmacological indexes and the information of HPLC fingerprint was discovered by multiple linear regression analysis and compound groups related to pharmacological indexes were obtained. Results: Antibacterial effect fingerprint of L. japonica can be established by HPLC fingerprint of ethyl acetate fraction. Conclusion: The method developed is practical and can be used for evaluating the quality control of L. japonica powder.