Rapid chemome profiling of Cistanche salsa using DI-MS/MS~(ALL) / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4150-4156, 2021.
Article
em Zh
| WPRIM
| ID: wpr-888075
Biblioteca responsável:
WPRO
ABSTRACT
The current study aims to rapidly and comprehensively profile the chemical composition of Cistanche salsa using direct infusion coupled with MS/MS~(ALL)(DI-MS/MS~(ALL)). The C. salsa extract was directly imported into electrospray ionization(ESI) source of quadrupole time-of-flight(Q-TOF) mass spectrometer with an infusion pump at a flow rate of 10 μL·min~(-1). Acquisition program was applied under negative ionization polarity to collect one MS~1 spectrum(m/z 50-1 200), followed by 1 150 MS~2 spectra with precursor isolation window(m/z 1) amongst mass range m/z 50-1 200. After each MS~2 spectrum was matched to its precursor ion, putative identification was conducted through matching mass spectral data with literature and database. A total of 31 components were identified from C. salsa, including 9 phenylethanoid glycosides, 2 iridoids, 4 saccharides, 9 organic acids, and 7 other compounds, similar to those from C. tubulosa and C. deserticola. In conclusion, DI-MS/MS~(ALL), a facile and reliable analytical tool, can be employed for qualitative analysis of chemical constituents in C. salsa. The research offers a promising strategy to achieve rapid chemome profiling of herbal medicine and provides an alternative source of Cistanches Herba.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Plantas Medicinais
/
Medicamentos de Ervas Chinesas
/
Cromatografia Líquida de Alta Pressão
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Cistanche
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Espectrometria de Massas em Tandem
/
Glicosídeos
Tipo de estudo:
Qualitative_research
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2021
Tipo de documento:
Article