Kinetics of dextran-independent α-(1→3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I.

Glucosyltransferase (GTF)-I from cariogenic Streptococcus sobrinus elongates the α-(1→3)-linked glucose polymer branches on the primer dextran bound to the C-terminal glucan-binding domain. We investigated the GTF-I-catalyzed glucan synthesis reaction in the absence of the primer dextran. The time course of saccharide production during dextran-independent glucan synthesis from sucrose was analyzed. Fructose and glucose were first produced by the sucrose hydrolysis. Leucrose was subsequently produced, followed by insoluble glucan [α-(1→3)-linked glucose polymers] after a lag phase. High levels of intermediate nigerooligosaccharide series accumulation were characteristically not observed during the lag phase. The results from the enzymatic activity of the acceptor reaction for the nigerooligosaccharide with a degree of polymerization of 2-6 and methyl α-D-glucopyranoside as a glucose analog indicate that the activity increased with an increase in the degree of polymerization. The production of insoluble glucan was numerically simulated using the fourth-order Runge-Kutta method with the kinetic parameters estimated from the enzyme assay. The simulated time course provided a profile similar to that of experimental data. These results define the relationship between the kinetic properties of GTF-I and the time course of saccharide production. These results are discussed with respect to a mechanism that underlies efficient glucan synthesis.

[Suppression of experimental inflammation by anti-fungal agent liranaftate in mice].

To evaluate the anti-inflammatory activity of the thiocarbamate antifungal agent liranaftate, the edema and the neutrophil accumulation detected by the activity of neutrophil marker enzyme, myeloperoxidase (MPO), were examined following application of liranaftate to mouse ears with inflammation induced by phorbol 12-myristate 13-acetate (PMA). Topical 20 microl administration of liranaftate in a dose-range between 1-4% suppressed the increase in ear thickness 6 hr after PMA application dose-dependently. Similarly, it decreased the weight increase of an ear section after 24 hr dose-dependently. More than 1% of liranaftate also suppressed augmentation of MPO activity of the ear section. This and histological observation indicate that liranaftate treatment suppressed neutrophil accumulation in PMA-applied ear lesion. From these results, we discussed that liranaftate might suppress inflammatory symptoms caused by trychophytosis in a clinical condition.

Suppression of anti-Candida activity of macrophages by a quorum-sensing molecule, farnesol, through induction of oxidative stress.

Farnesol is well known as a quorum-sensing molecule of Candida albicans. To assess the pathological function of farnesol, its effects on macrophage viability and functions including growth inhibitory activities against C. albicans were examined in vitro. Murine macrophages, when cultured in the presence of 56-112 microM of farnesol for 1-2 hr, decreased their activity inhibiting the mycelial growth of C. albicans and lost their viability. This suppression of macrophage function by farnesol was neutralized by the coexistence of the anti-oxidants probucol and trolox. Macrophages cultured in the presence of farnesol for 2 hr displayed morphological change of nuclei and DNA fragmentation, which suggested apoptosis of the cells. Intracellular production of ROS in the farnesol-treated macrophages was shown by fluorescence of DCFH-DA and increase of peroxidized materials. These effects of farnesol were blocked by probucol or trolox. These results indicate that farnesol lowered viability of the murine macrophages and suppressed their anti-Candida activity, perhaps through induction of ROS.

Prevalence and risk factors of urinary incontinence and its influence on the quality of life of Japanese women.

Urinary incontinence is a common problem as women age. The aims of this study were to assess the prevalence and risk factors of urinary incontinence and their impact on the quality of life of working Japanese women. In this cross-sectional study, 975 women completed the Urogenital Distress Inventory-6, the Short Form (SF)-36 Health Survey, and the King's Health Questionnaire. Their mean age was 47.6 years and the reported prevalence of stress, urge, and mixed urinary incontinence symptoms during the past month was 19.3%, 4.2%, and 7.8%, respectively. The prevalence rate of all the three types of incontinence increased with age and Body Mass Index. There was no significant difference in the questionnaire subscale scores of the SF-36 Health Survey and the King's Health Questionnaire between these types and those without urinary incontinence. However, in relation to the SF-36 scores, we found that urge urinary incontinence had more effect on women than stress urinary incontinence. There was a high prevalence of urinary incontinence in the participants. The results emphasize the need for women to be provided with education programs about incontinence and to perform preventive exercises.

[Volunteers' recognition of problems in meal distribution service and their expectation for information concerning health and welfare in less-favoured areas].

OBJECTIVE: This study aimed to clarify problems perceived by volunteers of a meal distribution service (MDS) in Less-Favoured Areas and the content of information expected to be provided by the administration and social welfare council. METHODS: We chose three municipalities (A, B, and C) with a population of less than 4,000 and an elderly population rate of 25% or higher. Volunteers involved in MDS in the municipalities were 28, 11, and 22 people in A, B, and C, respectively. We interviewed each group after explaining our purpose in writing, and made word-for-word records. Survey items were as follows; what you try and aim to do, what is your burden and pleasure, what are your problems and the solutions, and what are your demands for future activity in the MDS. The study period was from March to November, 2003. RESULTS: The information that the volunteers expected the administration and social welfare council to provide encompassed 3 categories, each of which contained 2 subcategories. They expected (information to allow elderly people to use the service on their own initiative> and as [information to improve understanding of the users and residents about the aims and contents of the MDS], and as [information to ensure the quality and quantity of volunteers], and and as [information to improve the relationships between the volunteers and users]. CONCLUSIONS: The volunteers expected education to improve the understanding of the residents so that elderly people can use the MDS on their own initiative, recruiting of motivated volunteers to ensure continuation of the service, and support for the smooth execution of the service and a system for such support. These were perceived by the subjects as problems to be solved for effective implementation of the service, and are considered to represent the meaning and state of the MDS that the volunteers wanted to share with the administration, social welfare council, and residents. The information that should be shared among the administration, social welfare council, residents, and volunteers can be clarified by evaluation of the expectations of the volunteers which must be met to promote their cooperation to support the service.