Preliminary investigation into the housing of dwarf hamsters.

BACKGROUND: Although pet hamsters are widely available, the literature on their husbandry and health is limited, and guidelines for the care of hamsters are variable. This study investigated housing in different dwarf hamster species, focusing on factors affecting co-housing failure. METHODS: An online cross-sectional survey shared on social media investigated potential factors influencing co-housing failure of dwarf hamsters in captivity, such as sex, species and resources provided. RESULTS: The majority of hamsters had not received veterinary care and were provided with fewer food and water sources than animal numbers, while 12% were kept in enclosures less than 75 cm in length. Co-housing failure was more likely in dwarf hamsters older than 6 months (odds ratio 4.75, p = 0.005, 95% confidence interval = 1.607-14.13). There was no association found between sex or species and co-housing failure. LIMITATIONS: There was likely a degree of selection bias as the owners who completed the questionnaire may have had more knowledge of dwarf hamster husbandry than the general population of owners. CONCLUSION: Key welfare concerns regarding dwarf hamster care in captivity were identified as inadequate enclosure sizes, a high prevalence of co-housing failure arising from aggression, especially in hamsters more than 6 months old, and inadequate resource provision for many pair- or group-housed animals. Further investigation of the welfare impacts of resource provision deficits is required.

Individual Monitoring of Activity and Lameness in Conventional and Slower-Growing Breeds of Broiler Chickens Using Accelerometers.

Accelerometers are increasingly being investigated to detect animal behavior as a method for monitoring individual welfare that overcomes manual challenges associated with time, resource, and discrete sampling. We investigated the effects of broiler chicken hybrid (hereafter breed) and weight on accelerometer activity (activityA; calculated as percentage of time spent active (%)) and its association with lameness as a major broiler welfare concern. Accelerometers were attached to birds of different breeds on between 2 and 4 occasions from 26 to 30 days old (conventional breed CNV) and 26 to 49 days old (two slower-growing breeds SGH; SGN). At 2.2 kg, lameness was scored using a 6-point gait scoring system (0: unaffected to 5: severely lame). Linear mixed effects models and breed-stratified generalized linear models together with a random-effect meta-analysis were used for data analyses. ActivityA was lower in faster-growing, heavier birds compared to slower-growing, lighter birds, showing overall consistency with previous behavioral research, but did not vary linearly with gait score. Accelerometers offer the potential for simple broad-scale continuous monitoring of broiler chicken activity behavior that requires limited data processing. Exploration of the ability of accelerometers to capture more subtle and specific changes in behavioral patterning, such as non-linear acceleration with gait score that could indicate early development of lameness, warrants further investigation.

The effect of visitors on the behavior of zoo-housed western lowland gorillas (Gorilla gorilla gorilla).

Primates, especially apes, are popular with the public, often attracting large crowds. These crowds could cause behavioral change in captive primates, whether positive, neutral, or negative. We examined the impact of visitors on the behavior of six western lowland gorillas (Gorilla gorilla gorilla), observing the troop over 6 weeks during high season (4.5 hr/day, 35 days, May-July 2016). We used focal scan sampling to determine activity budget and enclosure usage, and focal continuous sampling to identify bouts of anxiety-related behavior (visitor-directed vigilance, self-scratching, and aggression). Both daily zoo-entry numbers (VGATE ) and instantaneous crowds at the exhibit (VDENSITY ) were measured. Overall, VGATE had little effect across behaviors. However, consistent with the more acute time frame of measurement, VDENSITY was a better predictor of behavior; at high crowd volumes, we observed significant group-level changes in activity budget (increased inactivity, increased locomotion, and decreased environment-related behaviors), increase in some anxiety-related behaviors, and decreased enclosure usage. Although contributing similar effects, it could not be determined if crowd numbers, composition, or noise most affected the troop, nor any chronic effects of exposure to large crowds. Nevertheless, our findings suggest that measures to minimize the impacts of large crowds at the exhibit would be beneficial. Furthermore, we highlight potential discrepancies between common methods for measuring visitor numbers: VGATE is less sensitive to detecting visitor effects on behavioral indices than VDENSITY . Future studies should appropriately match the biological time frame of welfare indicators and visitor measures used to ensure the reliability of findings.

Reporting of suspected dog fighting to the police, Royal Society for the Prevention of Cruelty to Animals and equivalents by veterinary professionals in the UK.

Dog fighting became unlawful in the UK in 1835, yet it continues today (as reported by the Royal Society for the Prevention of Cruelty to Animals (RSPCA) and Crown Prosecution Service), although with an unknown prevalence. We used an online questionnaire to (1) determine the occurrence of dogs suspected of use in fighting in UK veterinary practices; (2) explore relative reporting of incidents to police, RSPCA or equivalent charity by registered veterinary nurses (RVN) and veterinarians; and (3) determine factors influencing reporting. Emails (n=2493) containing the questionnaire were sent to UK veterinary practices: 423 questionnaires (159 by RVNs, 264 by veterinarians) were completed. One or more cases of dog fighting were suspected by 14.4 per cent of respondents in 2015; 182 cases suspected in total. Proportionately more RVNs suspected dog fighting than veterinarians (P=0.0009). Thirty-two respondents (7.6 per cent, n=422) claimed to have reported suspicions to the police, the RSPCA or equivalent charity previously; 59 respondents (14.2 per cent) had previously chosen not to. Reasons not to report included: uncertainty of illegal activity (81.4 per cent), fear of the client not returning to the practice (35.6 per cent) and concerns regarding client confidentiality (22.0 per cent). Further work is required to address under-reporting of dog fighting by veterinary professionals.

Development and initial validation of a sensory threshold examination protocol (STEP) for phenotyping canine pain syndromes.

OBJECTIVE: To study the feasibility and test-retest repeatability of a sensory threshold examination protocol (STEP) and report the quantitative sensory threshold distributions in healthy dogs. STUDY DESIGN: Prospective, observational, cohort study. ANIMALS: Twenty-five healthy client-owned dogs. METHODS: Tactile sensitivity test (TST) (von Frey filaments), mechanical thresholds (MT with 2, 4 and 8 mm probes), heat thresholds (HT) and responsiveness to cold stimulus (CT at 0 °C) were quantitatively assessed for five body areas (BAs; tibias, humeri, neck, thoracolumbar region and abdomen) in a randomized order on three different occasions. Linear mixed model and generalized linear mixed models were used to evaluate the effects of body weight category, age, sex, BA, occasion, feasibility score and investigator experience. Test-retest repeatability was evaluated with the intra-class correlation coefficient. RESULTS: The STEP lasted 90 minutes without side effects. The BA affected most tests (p ≤ 0.001). Higher thresholds and longer cold latencies were scored in the neck (p ≤ 0.024) compared to other BAs. Weight category affected all thresholds (p ≤ 0.037). Small dogs had lower MT (∼1.4 N mean difference) and HT (1.1 °C mean difference) than other dogs (p ≤ 0.029). Young dogs had higher HT than adults (2.2 °C mean difference) (p = 0.035). Gender also affected TST, MT and HT (p < 0.05) (females versus males: TST odds ratio = 0.5, MT = 1.3 N mean difference, HT = 2.2 °C mean difference). Repeatability was substantial to moderate for all tests, but poor for TST. There was no difference in thresholds between occasions, except for CT. Test-retest repeatability was slightly better with the 2 mm MT probe compared to other diameters and improved with operator experience. CONCLUSIONS AND CLINICAL RELEVANCE: The STEP was feasible, was well tolerated and showed substantial test-retest repeatability in healthy dogs. Further validation is needed in dogs suffering pain.

Questionnaire-based behaviour analysis of Cavalier King Charles spaniels with neuropathic pain due to Chiari-like malformation and syringomyelia.

Chiari-like malformation (CM)/syringomyelia (SM) is a disease complex recognised in Cavalier King Charles spaniels (CKCSs) that can lead to neuropathic pain (NeP). In humans, NeP is associated with anxiety, depression and reduced quality of life (QoL). In this study, databases of three specialist veterinary centres were searched and CKCS breed societies and health forums were contacted to identify CKCS with an imaging diagnosis of CM/SM. Owners completed questionnaires on behaviour, signalment, general health status, NeP and QoL. Data were analysed from 122 dogs out of 564 questionnaires completed, after incomplete questionnaires and data from dogs that had other potentially debilitating disease processes were excluded. NeP severity score was significantly and positively correlated with 'stranger-directed' fear (r(S)=0.28), non-social fear (r(S)=0.34), 'separation-related' behaviour (r(S)=0.38), attachment behaviour (r(S)=0.24), excitability (r(S)=0.21) and proxy for pain sensation (r(S)=0.29). Increased NeP was also significantly associated with decreased QoL (r(S)=0.47), ability to settle (r(S)=0.26) and willingness to exercise (r(S)=0.50). Severity of NeP was positively associated with certain fear-associated behaviour and with decreased owner-perceived QoL. Thus, neurobehavioural changes should be considered in the management of NeP in CKCS with CM/SM.

Estimation of the number and demographics of companion dogs in the UK.

BACKGROUND: Current estimates of the UK dog population vary, contain potential sources of bias and are based on expensive, large scale, public surveys. Here, we evaluate the potential of a variety of sources for estimation and monitoring of the companion dog population in the UK and associated demographic information. The sources considered were: a public survey; veterinary practices; pet insurance companies; micro-chip records; Kennel Club registrations; and the Pet Travel Scheme. The public survey and subpopulation estimates from veterinary practices, pet insurance companies and Kennel Club registrations, were combined to generate distinct estimates of the UK owned dog population using a Bayesian approach. RESULTS: We estimated there are 9.4 (95% CI: 8.1-11.5) million companion dogs in the UK according to the public survey alone, which is similar to other recent estimates. The population was judged to be over-estimated by combining the public and veterinary surveys (16.4, 95% CI: 12.5-21.5 million) and under-estimated by combining the public survey and insured dog numbers (4.8, 95% CI: 3.6-6.9 million). An estimate based on combining the public survey and Kennel Club registered dogs was 7.1 (95% CI: 4.5-12.9) million. Based on Bayesian estimations, 77 (95% CI: 62-92)% of the UK dog population were registered at a veterinary practice; 42 (95% CI: 29-55)% of dogs were insured; and 29 (95% CI: 17-43)% of dogs were Kennel Club registered. Breed demographics suggested the Labrador was consistently the most popular breed registered in micro-chip records, with the Kennel Club and with J. Sainsbury's PLC pet insurance. A comparison of the demographics between these sources suggested that popular working breeds were under-represented and certain toy, utility and miniature breeds were over- represented in the Kennel Club registrations. Density maps were produced from micro-chip records based on the geographical distribution of dogs. CONCLUSIONS: A list containing the breed of each insured dog was provided by J. Sainsbury's PLC pet insurance without any accompanying information about the dog or owner.

Stimulus luminance and the spatial acuity of domestic fowl (Gallus g. domesticus).

The luminance dependence of spatial acuity in domestic fowl was measured directly over stimulus luminances ranging from 0.06 to 57.35 cd m(-2). At the highest luminance, acuity was around 6.5 c deg(-1), in agreement with previous studies in this species. As stimulus luminance decreased, acuity fell with increasing rate to 3.2 c deg(-1) at 0.06 cd m(-2), following the same shape as acuity functions for other mammalian and avian species. These findings suggest that the rod-cone transition for domestic fowl is between 0.45 and 1.79 cd m(-2). Over the photopic range from 1.79 to 57.35 cd m(-2) the change of acuity for fowl was 1%, compared with 32% for humans. For domestic fowl, the Rovamo-Barten MTF model of contrast sensitivity accounted for the behaviour of acuity as a function of luminance down to mesopic levels.

Measuring and modelling the spatial contrast sensitivity of the chicken (Gallus g. domesticus).

The spatial contrast sensitivity (CSF) of the chicken has been measured using a behavioural technique. The results obtained show that spatial vision in this species is relatively poor compared with the human observer. For a visual stimulus luminance of 16 c dm(-2), the upper frequency limit of spatial vision in the chicken (acuity) was found to be about 7.0 c deg(-1), with peak spatial vision occurring at around 1.0 c deg(-1). Under equivalent stimulus conditions, the acuity of the human is around 50 c deg(-1) with a peak in spatial vision at about 3.0 c deg(-1). Peak spatial contrast sensitivity in the chicken was also found to be only about 2% that for the human. At a lower stimulus luminance of 0.1 c dm(-2), the chicken CSF reduced in overall magnitude and indicated an acuity level of about 5.0 c deg(-1). These experimental results were successfully modelled using modulation transfer (MTF) theory. This theoretical treatment enabled important neural mechanisms underlying spatial vision in the chicken to be revealed. The role played by spatial vision in the chicken's ability to recognise detailed shapes in its visual environment was also examined by deploying the CSF as a visual weighting function with the Fourier series of a chicken comb.

Investigating social discrimination of group members by laying hens.

Social relationships in domestic fowl are commonly assumed to rely on social recognition and its pre-requisite, discrimination of group-mates. If this is true, then the unnatural physical and social environments in which commercial laying hens are typically housed, when compared with those in which their progenitor species evolved, may compromise social function with consequent implications for welfare. Our aims were to determine whether adult hens can discriminate between unique pairs of familiar conspecifics, and to establish the most appropriate method for assessing this social discrimination. We investigated group-mate discrimination using two learning tasks in which there was bi-directional exchange of visual, auditory and olfactory information. Learning occurred in a Y-maze task (p<0.003; n=7/8) but not in an operant key-pecking task (p=0.001; n=1/10). A further experiment with the operant-trained hens examined whether failure was specific to the group-mate social discrimination or to the response task. Learning also failed to occur in this familiar/unfamiliar social discrimination task (p=0.001; n=1/10). Our findings demonstrate unequivocally that adult laying hens kept in small groups, under environmental conditions more consistent with those in which sensory capacities evolved, can discriminate group members: however, appropriate methods to demonstrate discrimination are crucial.

Development of a raceway method to assess aversion of domestic fowl to concurrent stressors.

The requirement for assessing the effects of stressor combinations in improving the welfare of animals has not been widely recognised. Knowledge of the effects of concurrent stressors is needed to improve environments such as transport, where animals are presented with many simultaneous challenges. However, no method for measuring the effects of different stressors with a common unit is currently available. A locomotor passive avoidance method was developed as a common currency measure of the aversion of domestic fowl to concurrent stressors, using vibrational and thermal stressors as an exemplar. Juvenile fowl, fasted overnight, were trained to run a raceway into a goal-box for small food rewards (FR1). When running consistently, the reinforcement schedule was superimposed with a FR5 treatment schedule (60 min confinement in the goal-box with either a control of no other stressors [N] or concurrent vibration and thermal stressors [VT]). Subsequent latency to return to the goal-box was recorded as a measure of aversion. The factors affecting bird response were addressed in a series of experiments to optimise the method and clarify interpretation of results. Pre-feeding (20% ration 2 h prior to testing) did not affect response, but increasing the number of treatment presentations facilitated learning and increased method sensitivity. Treatment responses were consistent across experiments; overall VT was avoided (P<0.001), but N was not. However, there was large individual variation in response to VT. A final experiment indicated that, given a visual discriminatory cue, birds were capable of learning the required association between entering the goal-box and receiving the treatment, suggesting that the delay responses were due to aversion rather than the immediate impact of treatment on ability to respond. Further work is required to test the singular stressors, but the method retains common currency potential for assessing aversion to multiple stressors.