Performance analysis of a compact auto-phoropter for accessible refractive assessment of the human eye.

We present the performance analysis and specifications of a portable auto-phoropter system that can be employed for fast refractive assessment of a large population. A customized Shack-Hartmann wavefront sensor is developed to accurately measure the defocus and astigmatism of the eye within ±10D and ±6D, respectively. Three fluidic lenses are designed to correct the vision in real time. A digital Snellen chart is integrated into the system to validate the accuracy of the measurement and the correction by means of achieving 20/20 vision. The refractive error of eight subjects (16 eyes) has been measured objectively (without patient's feedback) using the proposed system and the results are compared with their clinical prescription through the Bland-Altman method. It is shown that the auto-phoropter takes less than 8 s to measure and correct the eye refractive error with an accuracy of ±0.25D.

Parametric dog-bone-shaped tunable cylindrical fluidic lens.

Tunable spherical fluidic lenses are among the most essential components in adaptive optics. However, fabricating cylindrical tunable lenses has proven more challenging, mainly due to the difficulty in eliminating the defocus component. We demonstrate a parametric approach to minimize the defocus in cylindrical tunable fluidic lenses. We theoretically model and experimentally verify that a dog-bone-shaped tunable cylindrical fluidic lens exhibits almost pure cylindrical performance within the range of ${\pm{\rm 5D}}$ of astigmatism. We anticipate these results will facilitate the use of tunable cylindrical fluidic lenses in adaptive optics applications and particularly ophthalmic devices, where rapid and reliable wavefront correction is required.

Single-cavity dual-wavelength all-fiber femtosecond laser for multimodal multiphoton microscopy.

A single-cavity dual-wavelength all-fiber femtosecond laser is designed to generate 1030 nm wavelength for high resolution multiphoton imaging and 1700 nm wavelength for long penetration depth imaging. Considering two-photon and three-photon microscopy (2PM and 3PM), the proposed laser provides the single-photon wavelength equivalent to 343 nm, 515 nm, 566 nm and 850 nm, that can be employed to excite a wide variety of intrinsic fluorophores, dyes, and fluorescent proteins. Generating two excitation wavelengths from a single laser reduces the footprint and cost significantly compared to having two separate lasers. Furthermore, an all-reflective microscope is designed to eliminate the chromatic aberration while employing two excitation wavelengths. The compact all-fiber alignment-free laser design makes the overall size of the microscope appropriate for clinical applications.

Compact fiber-based multi-photon endoscope working at 1700 nm.

We present the design, implementation and performance analysis of a compact multi-photon endoscope based on a piezo electric scanning tube. A miniature objective lens with a long working distance and a high numerical aperture (≈ 0.5) is designed to provide a diffraction limited spot size. Furthermore, a 1700 nm wavelength femtosecond fiber laser is used as an excitation source to overcome the scattering of biological tissues and reduce water absorption. Therefore, the novel optical system along with the unique wavelength allows us to increase the imaging depth. We demonstrate that the endoscope is capable of performing third and second harmonic generation (THG/SHG) and three-photon excitation fluorescence (3PEF) imaging over a large field of view (> 400 µm) with high lateral resolution (2.2 µm). The compact and lightweight probe design makes it suitable for minimally-invasive in-vivo imaging as a potential alternative to surgical biopsies.