RESUMEN
Germin and Germin-like proteins (GLPs) are a class of plant proteins that are part of the Cupins superfamily, found in several plant organs including roots, seeds, leaves, and nectar glands. They play a crucial role in plant defense against pathogens and environmental stresses. Herein, this study focused on the promoter analysis of OsGLP12-3 in rice cultivar Swat-1 to elucidate its regulation and functions. The region (1863bp) of the OsGLP12-3 promoter from Swat-1 genomic DNA was amplified, purified, quantified, and cloned using Topo cloning technology, followed by sequencing. Further in silico comparative analysis was conducted between the OsGLP12-3 promoters from Nipponbare and Swat-1 using the Plant CARE database, identifying 24 cis-acting regulatory elements with diverse functions. These elements exhibited distinct distribution patterns in the 2 rice varieties. The OsGLP12-3 promoter revealed an abundance of regulatory elements associated with biotic and abiotic stress responses. Computational tools were employed to analyze the regulatory features of this region. In silico expression analysis of OsGLP12-3, considering various developmental stages, stress conditions, hormones, and expression timing, was performed using the TENOR tool. Pairwise alignment indicated 86% sequence similarity between Nipponbare and Swat-1. Phylogenetic analysis was conducted to explore the evolutionary relationship between the OsGLP12-3 and other plant GLPs. Additionally, 2 unique regulatory elements were modeled and docked, GARE and MBS to understand their hydrogen bonding interactions in gene regulation. The study highlights the importance of OsGLP12-3 in plant defense against biotic and abiotic stresses, supported by its expression patterns in response to various stressors and the presence of specific regulatory elements within its promoter region.
RESUMEN
Photocatalytic ozonation, which combines the effects of lighting and ozonation, has been shown to enhance the decolorization and degradation of organic pollutants in wastewater. Dye solutions with concentrations of 10 ppm for both methylene blue and methyl orange dyes were used. The influence of ozoneation on the performance of photocatalytic activity of TiO2 and ZnO nanoparticles for the removal of organic dyes from aqueous solutions was investigated. To evaluate their efficacy for the removal of methylene blue and methyl orange dyes from aqueous solutions, the photocatalysts were exposed to UV light for 90 min, with ozone supplied either intermittently or continuously by an SDBD cold plasma reactor. The photocatalysts utilized in this study were characterized using SEM and XRD techniques. The degree of color degradation was determined using UV-Vis spectroscopy. The results demonstrate that TiO2 and ZnO nanoparticles exhibit different degrees of photocatalytic activity for the two dyes. The addition of ozone was found to enhance both the color degradation and mineralization rates of the pollutants, with intermittent ozonation proving more effective than continuous ozonation. The most significant color degradation results were obtained using TiO2 nanoparticles with intermittent ozonation for methylene blue dye (97 %) and ZnO nanoparticles with intermittent ozonation for methyl orange dye (40 %). Overall, this study provides evidence that photocatalytic ozonation represents a promising technique for water treatment.
RESUMEN
III-Nitride bandgap and refractive index data are of direct relevance for the design of (In, Ga, Al)N-based photonic and electronic devices. The bandgaps and bandgap bowing parameters of III-nitrides across the full composition range are reviewed with a special emphasis on InxAl1-xN, where less consensus was reached in the literature previously. Considering the available InAlN data, including those recently reported for low indium contents, empirical formulae for InAlN bandgap and bandgap bowing parameter are proposed. Applying the generalised bandgap data, the refractive index dispersion data available in the literature for III-N alloys is fitted using the Adachi model. For this purpose, a formalism involving a parabolic dependence of the Adachi parameters on the dimensionless bandgap [Formula: see text] of the corresponding ternary alloys is used rather than one directly invoking the alloy composition.