Identification of Sutcliffiella horikoshii strains in an immunobiological pharmaceutical industry facility.

The pharmaceutical industry must comply with the requirements for good manufacturing practices to reduce inherent contamination risks in the production process. Bacillus and related genera are among the main bacterial isolated from clean areas, raw material, and products in the pharmaceutical industries, but the correct identification of these species is still a challenge. The aim of this study was to characterize by phenotyping, protein profiling, and 16S rRNA gene sequencing Sutcliffiellahorikoshii strains (n = 6) isolated from an immunobiological pharmaceutical facility, and to propose the reclassification of Bacillus tianshenii to the genus Sutcliffiella, and Sutcliffiella tianshenii sp. nov. The strains were characterized by VITEK®2, matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) using VITEK®MS, and 16S rRNA gene sequencing analysis. MALDI-TOF/MS did not identify any strains that were identified by 16S rRNA as S. horikoshii. VITEK®2 showed false-positive results, with misidentification as B. sporothermodurans (reclassified as Heyndrickxia sporothermodurans) and Geobacillus thermoleovorans. After MALDI-TOF/MS database expansion, with the creation of SuperSpectrum, the strains were correctly identified as S. horikoshii. This study is the first report of isolation of S. horikoshii strains from a pharmaceutical industry. More studies are necessary to better understand the ability of S. horikoshii to contaminate the environment and products.

A violenta emoção e a justificação do feminicídio no Brasil (1930­1939) / The violent emotion and justification of feminicide in Brazil (1930­1939)

O artigo discute percursos históricos dos discursos de justificação do feminicídio no Brasil e analisa argumentos que justificam a violência contra a mulher tanto na cobertura midiática de casos criminais de feminicídio, quanto no repertório do pensamento criminológico e das práticas judiciárias brasileiras. A pesquisa utilizou levantamento documental e estudo de caso. Teve como fontes a cobertura midiática de periódicos do Rio de Janeiro do ano de 1930 aos crimes de uxoricídio e fragmentos de um processo criminal. Os resultados indicam a presença do discurso de justificação da violência letal intencional contra mulher por meio do julgamento/culpabilização da vítima pela violência sofrida, apoiada na referência à suposta conduta sexual imoral/adúltera da mulher. O estudo do caso demonstra a transformação desse operador discursivo no julgamento da vítima e no julgamento da enfermidade mental, por meio da articulação do discurso médico psiquiátrico da doença mental com a justiça criminal. O artigo conclui que o silenciamento das violências letais intencionais contra as mulheres sustenta-se num processo de irresponsabilização a partir do qual a violência praticada pelo homem contra a mulher é normatizada. Tal processo opera não somente em nome de uma suposta defesa da honra, mas também pelo recurso ao prejuízo das faculdades mentais sob o conceito de privação dos sentidos e da inteligência

TLR4 mutation protects neurovascular function and cognitive decline in high-fat diet-fed mice.

BACKGROUND: Metabolic syndrome (MS) is defined as a low-grade proinflammatory state in which abnormal metabolic and cardiovascular factors increase the risk of developing cardiovascular disease and neuroinflammation. Events, such as the accumulation of visceral adipose tissue, increased plasma concentrations of free fatty acids, tissue hypoxia, and sympathetic hyperactivity in MS may contribute to the direct or indirect activation of Toll-like receptors (TLRs), specifically TLR4, which is thought to be a major component of this syndrome. Activation of the innate immune response via TLR4 may contribute to this state of chronic inflammation and may be related to the neuroinflammation and neurodegeneration observed in MS. In this study, we investigated the role of TLR4 in the brain microcirculation and in the cognitive performance of high-fat diet (HFD)-induced MS mice. METHODS: Wild-type (C3H/He) and TLR4 mutant (C3H/HeJ) mice were maintained under a normal diet (ND) or a HFD for 24 weeks. Intravital video-microscopy was used to investigate the functional capillary density, endothelial function, and endothelial-leukocyte interactions in the brain microcirculation. Plasma concentrations of monocyte chemoattractant protein-1 (MCP-1), adipokines and metabolic hormones were measured with a multiplex immunoassay. Brain postsynaptic density protein-95 and synaptophysin were evaluated by western blotting; astrocytic coverage of the vessels, microglial activation and structural capillary density were evaluated by immunohistochemistry. RESULTS: The HFD-induced MS model leads to metabolic, hemodynamic, and microcirculatory alterations, as evidenced by capillary rarefaction, increased rolling and leukocyte adhesion in postcapillary venules, endothelial dysfunction, and less coverage of astrocytes in the vessels, which are directly related to cognitive decline and neuroinflammation. The same model of MS reproduced in mice deficient for TLR4 because of a genetic mutation does not generate such changes. Furthermore, the comparison of wild-type mice fed a HFD and a normolipid diet revealed differences in inflammation in the cerebral microcirculation, possibly related to lower TLR4 activation. CONCLUSIONS: Our results demonstrate that TLR4 is involved in the microvascular dysfunction and neuroinflammation associated with HFD-induced MS and possibly has a causal role in the development of cognitive decline.

[The role of social integration in the consumption of fruit and vegetables among the elderly]. / Rol de la integración social en el consumo de frutas y verduras en la adultez mayor.

This study investigates the extent to which aspects of the social integration of the elderly are linked to higher levels of fruit and vegetable consumption. It involved a cross-sectional study based on data from the National Study of Dependency in the Elderly, with a sample of 3278 elderly individuals. The variables of social integration considered were: frequency of meeting with close relatives, other relatives and neighbors and friends in the last 12 months; participation in recreational activities and in community groups, in addition to housing arrangements and marital status. The number of servings of fruit and vegetables consumed per day was the dependent variable. Logistic regression analysis was then conducted. In the adjusted model, more frequent encounters with siblings, in-laws and nephews, participation in community groups or organizations, are factors that increase the possibility of consuming two or more servings of fruit and vegetables a day, versus 1 serving or none; the opposite was observed when living alone. The variables of social integration that can increase the possibility of attaining the recommendation of consumption of fruit and vegetables daily (5 or more) in the adjusted model are frequency of encounters with siblings, in-laws and nephews and living alone.

Se investiga la medida en que aspectos de la integración social de adultos mayores están vinculados con los niveles de consumo de frutas y verduras. Estudio transversal a partir de datos del Estudio Nacional de la Dependencia en las Personas Mayores, con muestra de 3.278 adultos mayores. Las variables de integración social fueron: frecuencia de encuentro con familiares cercanos, otros familiares y vecinos y amigos en los últimos 12 meses; participación en actividades recreativas y en grupos comunitarios, además de arreglos de vivienda y estado marital. El número de porciones de frutas y verduras consumidas al día fue la variable dependiente. Se realizó análisis de regresión logística ordinal. En el análisis ajustado, mayor frecuencia de encuentros con hermanos, cuñados y sobrinos, participación en grupos comunitarios son factores que aumentan la posibilidad de consumo de dos o más porciones de frutas y verduras al día frente a ninguna o 1 porción; efecto contrario presenta vivir solo. Mayor frecuencia de encuentros con hermanos, cuñados y sobrinos y vivir sólo aumentan la posibilidad de alcanzar la recomendación de consumo de frutas y verduras diaria (5 o más), en el modelo ajustado.

Lifting facial não cirúrgico com fios de polidioxanona: revisão de literatura / Non-surgical facelift with polydiaxanone threads: literature review

O objetivo deste estudo foi realizar uma revisão de literatura acerca do lifting facial não cirúrgico com fios de PDO, abordando os diferentes tipos, suas indicações, a degradação no organismo, pla no de inserção, contraindicações, vantagens e possíveis complicações. As buscas foram realizadas nas bases de dados Pubmed, Bireme, Scielo, Sumários de Revistas Brasileiras e BVS, selecionan do-se artigos relevantes disponibilizados entre 2005 e 2020. Sendo os descritores utilizados em português e inglês para busca: "Harmonização Facial", "Fios de Sustentação", "Lifting Facial". Após aplicação dos critérios de inclusão e exclusão, dos 44 artigos encontrados, foram selecionados 16 para esse estudo. O uso dos fios de sustentação facial consiste em um método minimamente inva sivo, indolor, de caráter ambulatorial, anestesia local e efeito imediato. Diante dos possíveis riscos que acompanham o lifting cirúrgico, as pessoas estão buscando por alternativas menos invasivas, como os fios de sustentação. Apesar de não poderem ser considerados como alternativa a cirurgia reparadora convencional, os pacientes muitas vezes acabam optando pelo lifting com fios por se sentirem mais seguros, mesmo com resultados mais modestos. Observou-se que a utilização dos fios de PDO tem demostrado eficácia no rejuvenescimento facial quando bem indicados, apesar da possibilidade de ocorrência de efeitos adversos, normalmente pequenos e passageiros... (AU)

The aim of this study is to perform a literature review about non-surgical facial lifting with PDS threads, addressing its variety of types, indications, organism degradation, insertion plan, contrain dications, advantages and possible complications.The researches were conducted in the Pubmed, Bi reme, Scielo, Sumários de Revistas Brasileiras and BVS data bases, selecting relevant articles available between 2005 and 2020. After applying the inclusion and exclusion criteria, of the 44 articles found, 16 were selected for this study. Being used the following descriptors in Portuguese and English for research purposes: "Facial Harmonization", "Supporti Threads", "Face Lift". The use of facial support threads consists in a minimally invasive, painless, outpatient method, local anesthesia and imme diate effect. Faced with the possible risks that accompany the surgical facelift, people are looking for less invasive alternatives, such as the support wires. Although they cannot be considered as an alternative to conventional reparative surgery, patients often end up opting for a facelift because they feel safer, even with more modest results. It was observed that the use of PDO threads has been shown to be effective in facial rejuvenation when well indicated, despite the possibility of adverse effects, usually small and transient... (AU)

Population biology and sexual dimorphism in the freshwater prawn Atya scabra (Decapoda: Atyidae) in the Contas River, Bahia, Brazil / Biología poblacional y dimorfismo sexual en la gamba de agua dulce Atya scabra (Decapoda: Atyidae) en el río Contas, Bahía, Brasil

Introduction: Crustaceans have economic and ecological importance and the role of caridean crustaceans in lentic environments is crucial to maintain the trophic structure. The species Atya scabra is occasionally associated with fisheries, especially in Northeast and Northern Brazil. Objective: To determine the population structure, reproductive period, patterns of growth and distribution of size classes among sexes in a population of the prawn A. scabra in the De Contas River, Bahia, Brazil. Methods: Individuals were sampled in a period of one year by passing sieves through marginal vegetation and using dragnets. In the laboratory, we recorded the sex of the individuals and measured them using a pachymeter with 0.001 mm precision. The dimensions measured were CL (carapace length), TL (total length) and CW (carapace width). In order to analyze data, linear models for the relationships CL × CW were constructed and the type of allometry for each sex was determined. Individuals were grouped into size classes of 5 mm intervals. A chi-square test (χ2) was used to test deviation from the 1:1 sex ratio. The proportion of ovigerous females was registered for each month to determine the breeding period. Results: A total of 517 individuals were collected, 328 were males and 186 females of which 169 were ovigerous. Males showed a polymodal distribution in size classes and were significantly larger than females, which presented a unimodal distribution in size classes. The observed growth patterns demonstrated that both sexes present negative allometry. However, sexual dimorphism was detected when comparing the growth parameters. Ovigerous females were sampled the entire year and size-frequency data demonstrated that recruitment also occurs throughout the year, which allowed us to classify their reproduction as continuous. Conclusions: Our data is essential to draw sustainable fishery management strategies. We suggest a minimum capture size of 70 mm and capture restrictions from May to August.

Introducción: Los crustáceos tienen importancia económica y ecológica y el papel de los crustáceos carideanos en los entornos lénticos es crucial para mantener la estructura trófica. La especie Atya scabra se asocia ocasionalmente con la pesca, especialmente en el noreste y norte de Brasil. Objetivo: Determinar la estructura de la población, período reproductivo, patrones de crecimiento y la distribución de las clases de tamaño entre los sexos en una población de gambas A. scabra en el río de Contas, Bahía, Brasil. Métodos: Se tomaron muestras de los individuos en un período de un año pasando tamices en la vegetación marginal y usando redes de arrastre (dragnets). En el laboratorio, registramos el sexo de los individuos y los medimos utilizando un paquímetro con precisión de 0.001 mm. Las dimensiones medidas fueron CL (longitud del caparazón), TL (longitud total) y CW (ancho del caparazón). Para analizar los datos, se construyeron modelos lineales para las relaciones CL × CW y se determinó el tipo de alometría para cada sexo. Los individuos se agruparon en clases de tamaño de intervalos de 5 mm. La prueba de chi-cuadrado (χ2) se utilizó para determinar desviaciones en la proporción de sexos 1:1. La proporción de hembras ovígeras se registró en cada mes para determinar el período de reproducción. Resultados: Se recolectaron un total de 517 individuos, 328 machos y 186 hembras, de las cuales 169 eran ovígeras. Los machos mostraron una distribución polimodal en las clases de tamaño y fueron significativamente más grandes que las hembras, las cuales presentaron una distribución unimodal en las clases de tamaño. Los patrones de crecimiento observados demostraron que ambos sexos presentan alometría negativa. Sin embargo, se detectó dimorfismo sexual al comparar los parámetros de crecimiento. Se tomaron muestras de hembras ovígeras durante todo el año y los datos de frecuencia de tamaño demostraron que el reclutamiento también se produce durante todo el año, lo que nos permitió clasificar la reproducción como continua. Conclusiones: Nuestros datos son esenciales para elaborar estrategias sostenibles de gestión pesquera. Sugerimos un tamaño mínimo de captura de 70 mm y restricciones de captura desde mayo hasta agosto.

Effects of quarantine on mental health of populations affected by Covid-19.

BACKGROUND: To prevent the spread of the The novel coronavirus (2019-nCOV), some classic public health measures are being taken, such as social distancing and quarantine METHOD: Studies were identified using large-circulation international journals found in two electronic databases: Scopus and Embase. RESULTS: The prolonged confinement is evidently related to psychological damage, considering that individuals would be subjected to stressors for a longer period of time . In some cases, these psychic losses lasted for many months after the end of this confinement CONCLUSIONS: It is necessary to develop and implement actions to minimize the population psychological distress in meeting the needs of the communities affected by COVID-19 Thus, as it occurred in China, it is necessary that the other affected nations promote the publication of regulations that guide the implementation of mental health services and the allocation of resources, in order to ensure that individuals are monitored by competent professionals, thus reducing the psychological damage that can be motivated by the social isolation period during the quarantine.

Diálogos com o Feminino: pelas ondas do rádio mulheres que fazem psicologia para mulheres / Dialogues with the Feminine: by radio waves women who do psychology for women / Diálogos con lo Femenino: por las ondas de radio, mujeres que hacen psicología para mujeres

A pesquisa Diálogos com o Feminino aborda a trajetória de mulheres na psicologia brasileira, buscando evidenciar as especificidades do saber psi que se dedicou às questões do feminino e produziu uma intervenção específica de psicologia feita por mulheres e para mulheres. Esta comunicação apresenta dados da primeira fase da pesquisa, constituindo uma análise de abordagem qualitativa, natureza aplicada, com objetivo exploratório e procedimento de análise documental sobre a contribuição da jornalista e mestre em psicologia social Irede Cardoso para a psicologia articulada ao movimento feminista brasileiro. O estudo parte da experiência do programa de rádio Elas por Elas, compreendendo-o como uma original intervenção sobre a questão da mulher, tanto pelo diálogo que propunha com a psicologia, quanto pela utilização de instrumentos de comunicação via rádio e telefone, assim como pela colocação de questões específicas da mulher de forma coletivizada. Em seguida, são discutidos elementos da biografia de Irede Cardoso, abordando sua trajetória e produção acadêmica. Os resultados demonstram que o saber psi estudado na pesquisa formou esforços para a coletivização da pauta feminista.(AU)

The Dialogues with Feminine research addresses the trajectory of women in Brazilian psychology, seeking to highlight the specificities of psychological knowledge that has been dedicated to feminine issues and has produced a specific intervention of psychology by women and for women. This communication presents data from the first phase of the research, constituting an analysis of qualitative approach, applied nature, with an exploratory objective and documental analysis procedure about the contribution of the journalist and master in social psychology Irede Cardoso for the psychology articulated to the Brazilian feminist movement. The study begins with the experience of the radio program "Elas por Elas", understanding it as an original intervention on the issue of women, both for the dialogue it proposed with psychology, and for the use of radio and telephone, as well as by placing specific questions of the woman collectively. Then, elements of the biography of Irede Cardoso are discussed, detailing their trajectory and academic production. The results demonstrate that the psychological knowledge studied in the research has made efforts for the collectivization of the feminist agenda.(AU)

La investigación Diálogos con lo Femenino aborda la trayectoria de mujeres en la psicología brasileña, buscando evidenciar las especificidades del saber psi que se dedicó a las cuestiones de lo femenino y produjo una intervención específica de psicología hecha por mujeres y para mujeres. Esta comunicación presenta datos de la primera fase de la investigación, constituyendo un análisis de abordaje cualitativo, de naturaleza aplicada, con objetivo exploratorio y procedimiento de análisis documental sobre la contribución de la periodista y magíster en psicología social Irede Cardoso para la psicología articulada al movimiento feminista brasileño. El estudio se inició con la experiencia del programa de radio Ellas por Ellas, comprendiéndolo como una intervención original sobre la cuestión de la mujer, tanto por el diálogo que proponía con la psicología, como por la utilización de instrumentos de comunicación vía radio y teléfono, así como por la colocación de cuestiones específicas de la mujer de forma colectiva. A continuación, se discuten elementos de la biografía de Irede Cardoso, detallando su trayectoria y producción académica. Los resultados demuestran que el saber psi estudiado en la investigación significó un esfuerzo para la colectivización de la pauta feminista.(AU)

Correspondence between try-in pastes and resin cements, and color stability of bonded lithium disilicate disks.

This study investigates the color correspondence of resin cements and try-in pastes, and the color stability of bonded lithium disilicate ceramic disks. Resin composite disks were fabricated (n = 36) to serve as the background for lithium disilicate disks prepared in two thicknesses (0.5 and 1.0 mm, n = 18 each). Two brands were used for try-in and cement procedures: Variolink Veneer and AllCem Veneer. For baseline, water was applied between the ceramic disks and their respective backgrounds to achieve the control group. This set was subjected to color measurement using an intraoral measurement device (T0). The try-in was inserted between background and ceramic, and this set was subjected to color measurement (T1). After adhesive procedures, the ceramic disk was placed under cement, and color measurement was performed with uncured cement (T2) and 24 h after light-curing (T3). Each set was immersed in distilled water and thermal-cycled, with color measurement being performed after 10,000 (T4) and 20,000 (T5) cycles. Color differences were calculated by CIELab (rEab) and CIEDE2000 (rE00). Data were analyzed by two-way ANOVA for repeated measurements and Tukey's test (α=5%). There was color correspondence of try-in and resin cement for the Variolink system, regardless of the ceramic thickness (p > 0.05). For the AllCem system, the thickness significantly influenced the color measurement (p < 0.001). The Variolink system also demonstrated color stability after 20,000 thermal cycles with rEab < 3.46 and rE00 < 2.25. It was concluded that the color correspondence between a try-in and its respective cement may vary according to resin cement composition.

Correspondence between try-in pastes and resin cements, and color stability of bonded lithium disilicate disks

Abstract: This study investigates the color correspondence of resin cements and try-in pastes, and the color stability of bonded lithium disilicate ceramic disks. Resin composite disks were fabricated (n = 36) to serve as the background for lithium disilicate disks prepared in two thicknesses (0.5 and 1.0 mm, n = 18 each). Two brands were used for try-in and cement procedures: Variolink Veneer and AllCem Veneer. For baseline, water was applied between the ceramic disks and their respective backgrounds to achieve the control group. This set was subjected to color measurement using an intraoral measurement device (T0). The try-in was inserted between background and ceramic, and this set was subjected to color measurement (T1). After adhesive procedures, the ceramic disk was placed under cement, and color measurement was performed with uncured cement (T2) and 24 h after light-curing (T3). Each set was immersed in distilled water and thermal-cycled, with color measurement being performed after 10,000 (T4) and 20,000 (T5) cycles. Color differences were calculated by CIELab (rEab) and CIEDE2000 (rE00). Data were analyzed by two-way ANOVA for repeated measurements and Tukey's test (α=5%). There was color correspondence of try-in and resin cement for the Variolink system, regardless of the ceramic thickness (p > 0.05). For the AllCem system, the thickness significantly influenced the color measurement (p < 0.001). The Variolink system also demonstrated color stability after 20,000 thermal cycles with rEab < 3.46 and rE00 < 2.25. It was concluded that the color correspondence between a try-in and its respective cement may vary according to resin cement composition.