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1.
Gen Dent ; 72(3): 70-73, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38640010

RESUMEN

Treatment of peri-implant diseases focuses on reducing the bacterial load and consequent infection control. The use of local antimicrobials as an adjunct to mechanical therapy may result in a better outcome. Among antimicrobials, doxycycline stands out because of its local modulation of cytokines, microbial reduction, and clinical parameters in the treatment of periodontal diseases. The objective of this case report was to describe the combined application of mechanical debridement and bioresorbable doxycycline-loaded nanospheres for the treatment of peri-implantitis in a 71-year-old man. At the 3-year evaluation, the peri-implant tissues had improved, showing decreased probing depths, an absence of bleeding on probing, and no suppuration. This case report highlights the importance of supportive therapy, which is essential for the long-term success of peri-implantitis treatment.


Asunto(s)
Antiinfecciosos , Implantes Dentales , Nanosferas , Periimplantitis , Masculino , Humanos , Anciano , Periimplantitis/tratamiento farmacológico , Periimplantitis/microbiología , Doxiciclina/uso terapéutico , Estudios de Seguimiento , Desbridamiento , Implantes Absorbibles , Antiinfecciosos/uso terapéutico , Resultado del Tratamiento
2.
Mater Sci Eng C Mater Biol Appl ; 119: 111638, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33321676

RESUMEN

Different crystalline phases in sputtered TiO2 films were tailored to determine their surface and electrochemical properties, protein adsorption and apatite layer formation on titanium-based implant material. Deposition conditions of two TiO2 crystalline phases (anatase and rutile) were established and then grown on commercially pure titanium (cpTi) by magnetron sputtering to obtain the following groups: A-TiO2 (anatase), M-TiO2 (anatase and rutile mixture), R-TiO2 (rutile). Non-treated commercially pure titanium (cpTi) was used as a control. Surfaces characterization included: chemical composition, topography, crystalline phase and surface free energy (SFE). Electrochemical tests were conducted using simulated body fluid (SBF). Albumin adsorption was measured by bicinchoninic acid method. Hydroxyapatite (HA) precipitation was evaluated after 28 days of immersion in SBF. MC3T3-E1 cell adhesion, morphology and spreading onto the experimental surfaces were evaluated by scanning electron microscopy. Sputtering treatment modified cpTi topography by increasing its surface roughness. CpTi and M-TiO2 groups presented the greatest SFE. In general, TiO2 films displayed improved electrochemical behavior compared to cpTi, with M-TiO2 featuring the highest polarization resistance. Rutile phase exhibited a greater influence on decreasing the current density and corrosion rate, while the presence of a bi-phasic polycrystalline condition displayed a more stable passive behavior. M-TiO2 featured increased albumin adsorption. HA morphology was dependent on the crystalline phase, being more evident in the bi-phasic group. Furthermore, M-TiO2 displayed normal cell adhesion and morphology. The combination of anatase and rutile structures to generate TiO2 films is a promising strategy to improve biomedical implants properties including greater corrosion protection, higher protein adsorption, bioactivity and non-cytotoxicity effect.


Asunto(s)
Prótesis e Implantes , Titanio , Microscopía Electrónica de Rastreo , Propiedades de Superficie , Difracción de Rayos X
3.
Mater Sci Eng C Mater Biol Appl ; 117: 111289, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32919650

RESUMEN

Our goal was to create bio-functional chlorhexidine (CHX)-doped thin films on commercially pure titanium (cpTi) discs using the glow discharge plasma approach. Different plasma deposition times (50, 35 and 20 min) were used to create bio-functional surfaces based on silicon films with CHX that were compared to the control groups [no CHX and bulk cpTi surface (machined)]. Physico-chemical and biological characterizations included: 1. Morphology, roughness, elemental chemical composition, film thickness, contact angle and surface free energy; 2. CHX-release rate; 3. Antibacterial effect on Streptococcus sanguinis biofilms at 24, 48 and 72 h; 4. Cytotoxicity and metabolic activity using fibroblasts cell culture (NIH-F3T3 cells) at 1, 2, 3 and 4 days; 5. Protein expression by NIH-F3T3 cells at 1, 2, 3 and 4 days; and 6. Co-culture assay of fibroblasts cells and S. sanguinis to assess live and dead cells on the confocal laser scanning microscopy, mitochondrial activity (XTT), membrane leakage (LDH release), and metabolic activity (WST-1 assay) at 1, 2 and 3 days of co-incubation. Data analysis showed that silicon films, with or without CHX coated cpTi discs, increased surface wettability and free energy (p < 0.05) without affecting surface roughness. CHX release was maintained over a 22-day period and resulted in a significant inhibition of biofilm growth (p < 0.05) at 48 and 72 h of biofilm formation for 50 min and 20 min of plasma deposition time groups, respectively. In general, CHX treatment did not significantly affect NIH-F3T3 cell viability (p > 0.05), whereas cell metabolism (MTT assay) was affected by CHX, with the 35 min of plasma deposition time group displaying the lowest values as compared to bulk cpTi (p < 0.05). Moreover, data analysis showed that films, with or without CHX, significantly affected the expression profile of inflammatory cytokines, including IL-4, IL-6, IL-17, IFN-y and TNF-α by NIH-F3T3 cells (p < 0.05). Co-culture demonstrated that CHX-doped film did not affect the metabolic activity, cytotoxicity and viability of fibroblasts cells (p > 0.05). Altogether, the findings of the current study support the conclusion that silicon films added with CHX can be successfully created on titanium discs and have the potential to affect bacterial growth and inflammatory markers without affecting cell viability/proliferation rates.


Asunto(s)
Clorhexidina , Titanio , Biopelículas , Clorhexidina/farmacología , Streptococcus sanguis , Propiedades de Superficie
4.
Braz Oral Res ; 33: e058, 2019 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-31432925

RESUMEN

Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.


Asunto(s)
Técnicas de Cultivo de Célula/métodos , Cemento Dental/citología , Proteínas Adaptadoras Transductoras de Señales , Adolescente , Adulto , Análisis de Varianza , Proteínas Morfogenéticas Óseas/análisis , Proteínas Morfogenéticas Óseas/genética , Línea Celular , Cemento Dental/metabolismo , Proteínas de la Matriz Extracelular/análisis , Proteínas de la Matriz Extracelular/genética , Femenino , Técnica del Anticuerpo Fluorescente , Expresión Génica , Marcadores Genéticos/genética , Humanos , Masculino , Glicoproteínas de Membrana/análisis , Glicoproteínas de Membrana/genética , Diente Molar/citología , Fosfatos/farmacología , Fosfoproteínas/análisis , Fosfoproteínas/genética , Sialoglicoproteínas/análisis , Sialoglicoproteínas/genética , Factores de Tiempo , Adulto Joven
5.
Bone ; 125: 128-139, 2019 08.
Artículo en Inglés | MEDLINE | ID: mdl-31077853

RESUMEN

Hypophosphatasia (HPP) is an inherited metabolic disorder that causes defective skeletal and dental mineralization. HPP exhibits a markedly heterogeneous range of clinical manifestations caused by dysfunction of the tissue-nonspecific isozyme of alkaline phosphatase (TNSALP), resulting from loss-of-function mutations in the ALPL gene. HPP has been associated with predominantly missense mutations in ALPL, and a number of compound heterozygous genotypes have been identified. Here, we describe a case of a subject with adult-onset HPP caused by a novel combination of missense mutations p.Gly473Ser and p.Ala487Val, resulting in chronic musculoskeletal pain, myopathy, persistent fatigue, vomiting, and an uncommon dental phenotype of short-rooted permanent teeth. Pedigree and biochemical analysis indicated that severity of symptoms was correlated with levels of residual ALP activity, and co-segregated with the p.Gly473Ser missense mutation. Bioinformatic analysis to predict the structural and functional impact of each of the point mutations in the TNSALP molecule, and its potential contribution to the clinical symptoms, revealed that the affected Gly473 residue is localized in the homodimer interface and predicted to have a dominant negative effect. The affected Ala487 residue was predicted to bind to Tyr479, which is closely located the N-terminal α-helix of TNSALP monomer 2, suggesting that both changes may impair dimer stability and catalytic functions. In conclusion, these findings assist in defining genotype-phenotype associations for HPP, and further define specific sites within the TNSALP molecule potentially related to neuromuscular manifestations in adult HPP, allowing for a better understanding of HPP pathophysiology.


Asunto(s)
Hipofosfatasia/genética , Hipofosfatasia/patología , Mutación/genética , Adulto , Fosfatasa Alcalina/genética , Secuencia de Aminoácidos , Biología Computacional , Femenino , Estudios de Asociación Genética , Humanos , Masculino , Datos de Secuencia Molecular , Linaje , Adulto Joven
6.
Braz. oral res. (Online) ; 33: e058, 2019. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1019608

RESUMEN

Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.


Asunto(s)
Humanos , Masculino , Femenino , Adolescente , Adulto , Adulto Joven , Marcadores Genéticos/genética , Técnicas de Cultivo de Célula/métodos , Cemento Dental/citología , Fosfatos/farmacología , Fosfoproteínas/análisis , Fosfoproteínas/genética , Sialoglicoproteínas/análisis , Sialoglicoproteínas/genética , Factores de Tiempo , Glicoproteínas de Membrana/análisis , Glicoproteínas de Membrana/genética , Expresión Génica , Línea Celular , Análisis de Varianza , Proteínas de la Matriz Extracelular/análisis , Proteínas de la Matriz Extracelular/genética , Técnica del Anticuerpo Fluorescente , Proteínas Morfogenéticas Óseas/análisis , Proteínas Morfogenéticas Óseas/genética , Cemento Dental/metabolismo , Proteínas Adaptadoras Transductoras de Señales , Diente Molar/citología
7.
ImplantNewsPerio ; 2(6): 1085-1091, nov.-dez. 2017. ilus
Artículo en Portugués | LILACS, BBO | ID: biblio-880971

RESUMEN

A retração gengival é caracterizada como o deslocamento apical da margem gengival em relação à junção cemento/esmalte (JCE), possuindo como consequência a exposição da superfície radicular. Diversas técnicas já foram descritas na literatura científi ca como tratamento para este problema gengival e, geralmente, a técnica cirúrgica de recobrimento radicular a ser escolhida relaciona-se com as características clínicas do caso, preferência do operador, habilidade manual, classifi cação da retração gengival, dentre outros. No entanto, a literatura indica que, dentre os procedimentos disponíveis, a técnica de retalho posicionado coronalmente com o uso de enxerto de tecido conjuntivo subepitelial parece ser a mais promissora. O objetivo deste trabalho foi apresentar um relato de caso de recobrimento radicular, utilizando a técnica de Zucchelli & De Sanctis associada a enxerto de tecido conjuntivo para recobrimento de retrações gengivais múltiplas. Paciente do sexo feminino, com 24 anos de idade, sem relato de alterações sistêmicas e/ou alergias, não etilista e não fumante, procurou tratamento odontológico queixando-se de hipersensibilidade dentinária e comprometimento estético bucal. Os resultados observados permitiram concluir que a técnica descrita por Zucchelli & De Sanctis, associada ao enxerto de tecido conjuntivo subepitelial, permitiu reduzir a altura das retrações gengivais, assim como aumentar e melhorar a qualidade do tecido gengival, com completa resolução da hipersensibilidade dentinária e alto nível de satisfação estética.


Gingival retraction is characterized as the apical displacement of the gingival margin in relation to the cementitious enamel junction (JCE), with consequent exposure to the root surface. Several techniques have already been described in the scientifi c literature as treatment for this gingival problem, and the surgical technique of root coverage to be chosen is generally related to the clinical characteristics of the case, operator preference, manual ability, classifi cation of gingival retraction, among others. However, the literature indicates that among the available procedures, the coronally positioned fl ap technique with the use of subepithelial connective tissue graft seems to be the most promising technique. The objective of this work is to present a case report of root coverage using the Zucchelli & De Sanctis technique associated with a connective tissue graft to cover multiple gingival retractions. Patient 24 years old, female, with no reports of systemic alterations and/or allergies, non-alcoholic and non-smoker, sought dental treatment complaining of dentine hypersensitivity and aesthetic impairment. The results showed that the technique described by de Zucchelli & De Sanctis associated with the subepithelial connective tissue graft reduced the height of gingival retractions, as well as increase and improve gingival tissue quality, with complete resolution of dentin hypersensitivity and high level of aesthetic satisfaction.


Asunto(s)
Humanos , Femenino , Adulto , Tejido Conectivo/trasplante , Estética Dental , Recesión Gingival/cirugía , Procedimientos Quirúrgicos Orales , Cirugía Bucal/métodos , Trasplante de Tejidos/métodos
8.
ImplantNewsPerio ; 2(4): 747-752, jul.-ago. 2017.
Artículo en Portugués | LILACS, BBO | ID: biblio-860040

RESUMEN

O uso de implantes dentários é considerado uma revolução na Odontologia moderna. Entretanto, um dos grandes desafios encontrados na Implantodontia é o processo de reabsorção óssea ao redor dos implantes, após sua colocação ou durante o uso. Esta revisão teve por objetivo discutir alguns avanços nas pesquisas científicas que utilizam parâmetros clínicos para classificação da doença peri-implantar. Diversos estudos na literatura demonstram que essas ferramentas podem promover uma melhor compreensão, tanto do cirurgião-dentista quanto dos pacientes que necessitam da utilização desses.


The use of dental implants is considered a revolution in modern dentistry. However, the great challenges found at implantodontology is the process of bone resorption around implants after placement or during use. This review aims to discuss some advances in scientific research that use clinical parameters to classify peri-implant disease. Several studies in the literature demonstrate these tools can promote a better understanding for both dental surgeons and patients in need of this.


Asunto(s)
Humanos , Huesos , Regeneración Ósea , Resorción Ósea , Implantes Dentales , Periimplantitis
9.
ImplantNewsPerio ; 2(3): 495-505, mai.-jun. 2017. ilus
Artículo en Portugués | LILACS, BBO | ID: biblio-847263

RESUMEN

Embora tenha havido avanço no entendimento da homeostase do cemento dental, o papel deste tecido e sua biologia permanecem não completamente elucidados. Este estudo buscou fornecer informações sobre os conhecimentos mais recente relacionados à biologia do cemento dental, com o objetivo de discutir o papel exercido por este tecido em condições não fisiológicas nos tecidos periodontais. Devido aos avanços na exploração do tecido ósseo, que compartilha diversas características similares, a pesquisa abrangente sobre o cemento dental tem sido encorajada, a fim de esclarecer a função completa deste tecido na homeostase periodontal e regeneração. Desta forma, no presente trabalho, sempre que possível será feito um paralelo entre osso alveolar e cemento dental. O desenvolvimento de metodologias e técnicas celulares e moleculares avançadas possibilitou um melhor entendimento do comportamento do cemento em situações diversas, como quando em situações patológicas, como a doença periodontal, e até mesmo frente à regeneração tecidual. Ademais, estudos clínicos e em modelo animal demonstraram resultados em relação à formação de cemento em abordagens regenerativas. No entanto, sugere-se que estudos posteriores possam contribuir para um melhor conhecimento sobre o cemento e o perfil celular dos cementoblastos e cementócitos, bem como suas interações para fornecer novos insights para o desenvolvimento de terapias eficientes e mais previsíveis para regeneração dos tecidos periodontais. Apesar dos avanços dos estudos clínicos e laboratoriais, pôde-se concluir que inúmeras questões referentes à biologia do cemento permanecem não esclarecidas.


Although some progress has been made to understand dental cementum homeostasis, its role and biology remains not completely elucidated. This study aimed to provide information on the recent knowledge related to the dental cementum biology, in order to discuss the role of this tissue in physiological and non-physiological conditions in the periodontal tissues. Due to advances in the exploration of bone tissue, which shares several similar features, comprehensive research on dental cementum has been encouraged in order to clarify the complete function of this tissue in periodontal homeostasis and regenerative approach. Novel methodologies and advanced cellular and molecular techniques provided better understanding of cementum in different circumstances, as pathological situations such as periodontal disease and even tissue regeneration. In addition, clinical and animal model designs show positive outcomes to cementum formation in regenerative approaches, however, it is suggested that further studies may contribute to better understand cementum tissue and cementoblasts and cementocytes profile, as well as their interactions, providing new insights to develop efficient and more predictable therapies for periodontal tissue regeneration. Despite advances in clinical and laboratory studies, it can be concluded that many questions regarding the cementum biology remain unclear.


Asunto(s)
Humanos , Huesos , Regeneración Ósea , Cementogénesis , Cemento Dental/anatomía & histología , Cemento Dental/fisiología , Enfermedades Periodontales
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