RESUMEN
BACKGROUND: The purpose of work was development of a fast and reproduced procedure for measurement of the total complement activity (TCA) in human or animal blood serum. MATERIALS AND METHODS: Steady at storage liposomes preparations, which surface sensitized 2,4-DNP haptens, and the internal volume contains calceine or sulforhodamine 101 are obtained. Complement-dependent immune lysis of liposomes at presence of the anti-2,4-DNP immunoglobulines and complement preparations from animals are investigated. RESULTS: It is shown that the degree of liposomes immune lysis depends on complement concentration in a wide range that can be used for definition of TCA level. Research of blood sera from patients has revealed correlation (r = 0.793) between data received with the help of liposome immunolytic systems, and the data of nephelometric analysis with application of suspension sheep erythrocytes. CONCLUSION: The method allows to define total complement activity in blood serum in 15 minutes without separation of reaction components. This might be useful for measurement TCA level at patients with various diseases and realization of scientific researches.
Asunto(s)
Activación de Complemento/inmunología , Proteínas del Sistema Complemento/inmunología , Liposomas , 2,4-Dinitrofenol/análogos & derivados , Animales , Colorantes Fluorescentes , Haptenos/efectos de los fármacos , Humanos , Técnicas Inmunológicas/métodos , Liposomas/inmunologíaAsunto(s)
Antígenos/inmunología , Autoanticuerpos/sangre , Proteínas del Sistema Complemento , Insuficiencia de la Válvula Mitral/etiología , Estenosis de la Válvula Mitral/etiología , Miocardio/inmunología , Urticaria/complicaciones , Vasculitis/complicaciones , Adulto , Femenino , Humanos , Urticaria/sangre , Urticaria/inmunología , Vasculitis/sangre , Vasculitis/inmunologíaRESUMEN
The method is based on the ability of inulin to activate the alternative route of the complement system. The alternative activity is assessed from the consumption of the third complement component as a result of interaction with inulin. C3 is measured in experimental and control samples by kinetic titration, which permits assessing the activation of the alternative route in patients sera in less than 2 hours.
Asunto(s)
Vía Alternativa del Complemento , Complemento C1/análisis , Complemento C3/análisis , Vía Alternativa del Complemento/inmunología , Humanos , Inulina , Temperatura , Factores de TiempoRESUMEN
The method is intended for preparing a reagent for the detection of the second component and factor B of the complement system. It makes use of an unsophisticated device for even time-controlled heating of the serum by letting it pass through a coil pipe submerged in the bath. The resultant reagent is used to measure the activity of the second component and B factor of the complement system and may be used for assessing humoral immunity in patients.