RESUMEN
Listeria monocytogenes infection in pregnant women and newborns is a cause for serious concern, and invasive disease outcome strongly depends on prompt antibiotic therapy. To provide sooner identification from neonatal bacteremia we performed a CAMP test directly on positive blood aliquots and inoculated the Liofilchem(®) O.A. Listeria chromogenic agar as well, thus providing a 24-h turn-around time for response.
Asunto(s)
Bacteriemia/diagnóstico , Técnicas Bacteriológicas , Compuestos Cromogénicos , Diagnóstico Precoz , Listeriosis/diagnóstico , Agar , Humanos , Recién Nacido , Listeria monocytogenes/aislamiento & purificación , Factores de TiempoRESUMEN
A beta-hemolytic Lancefield antigen A-, B-, C-, D-, F-, and G-positive Enterococcus durans strain was cultivated from the rectovaginal swab of a pregnant woman who underwent antenatal screening for Streptococcus agalactiae. The isolate raised concern as to what extent similar strains are misrecognized and lead to false diagnosis of group B streptococci.
Asunto(s)
Enterococcus/aislamiento & purificación , Infecciones por Bacterias Grampositivas/diagnóstico , Antígenos Bacterianos/análisis , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Diagnóstico Diferencial , Femenino , Infecciones por Bacterias Grampositivas/microbiología , Hemólisis , Humanos , Datos de Secuencia Molecular , Embarazo , Diagnóstico Prenatal , ARN Ribosómico 16S/genética , Recto/microbiología , Análisis de Secuencia de ADN , Serotipificación , Streptococcus agalactiae/aislamiento & purificación , Vagina/microbiologíaRESUMEN
Enterococcus hirae is rarely collected from man, while it is a common pathogen in mammals and birds. We describe the first isolation of the organism (strain DSM 27815) from human umbilical cord blood (UCB), thus emphasizing the risk of contamination of UCB units for clinical use. In this context, we also highlight the importance of an extensive training of the collecting personnel as to the observance of the disinfection protocol ensuring UCB units sterility.
Asunto(s)
Bacteriemia/diagnóstico , Errores Diagnósticos , Enterococcus/aislamiento & purificación , Sangre Fetal/microbiología , Infecciones por Bacterias Grampositivas/diagnóstico , Animales , Bacteriemia/microbiología , Aves , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Enterococcus/clasificación , Infecciones por Bacterias Grampositivas/microbiología , Humanos , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
Corynebacterium striatum is often dismissed as a contaminant when cultivated from blood samples; indeed, it is a skin saprophyte that may therefore be introduced into the clinical specimen accidentally. Nevertheless, the organism can be responsible for true bacteraemias, and multidrug resistance spread among nosocomial strains is of increasing concern. Specific criteria for testing have not been defined yet, but we however suggest to report clear resistances (i.e. absence of any inhibition zones with the disc test), in order to try to understand this species behaviour under antibiotic exposure. In this context, features of a blood isolate (strain DSM 45711) are here depicted.