A high-efficient antibacterial and biocompatible polyurethane film with Ag@rGO nanostructures prepared by microwave-assisted method: Physicochemical and dermal wound healing evaluation.

Wound infections are a significant issue that can hinder the wound healing process. One way to address this problem is by enhancing the antibacterial activity of wound dressings. Accordingly, this work focuses on developing a castor-oil-based antibacterial polyurethane nanocomposite film impregnated with silver nanoparticles (AgNPs) decorated on the surface of reduced graphene oxide (rGO) nanostructures (Ag@rGO). To this aim, rGOs act as a platform to stabilize AgNPs and improve their bioavailability and dispersion quality within the PU film. The microwave-assisted synthesis of Ag@rGO nanohybrids was proved by FTIR, XRD, TGA, FE-SEM, EDS, and TEM analyses. Compared to PU/GO, the effect of Ag@rGO nanohybrids on thermo-mechanical features, morphology, antibacterial activity, cytocompatibility, and in vivo wound healing was assessed. SEM photomicrographs revealed the enhanced dispersion of Ag@rGO nanohybrids compared to GO nanosheets. Besides, according to XRD results, PU/Ag@rGO nanocomposite film demonstrated higher microphase mixing, which could be due to the finely dispersed Ag@rGO nanostructures interrupting the hydrogen bonding interactions in the hard segments. Moreover, PU/Ag@rGO nanocomposite showed excellent antibacterial behavior with completely killing E. coli and S. aureus bacteria. In vitro and in vivo wound healing studies displayed PU/Ag@rGO film effectively stimulated fibroblast cells proliferation, migration and re-epithelialization. However, the prepared antibacterial PU/Ag@rGO nanocomposite film has the potential to be used as a biomaterial for dermal wound healing applications.

Antibacterial Host-Guest Intercalated LDH-Adorned Polyurethane for Accelerated Dermal Wound Healing.

Curcumin has a limited clinical application because of its extremely poor accessibility. In the present study, improved curcumin bioavailability within a castor oil polyurethane/layered double hydroxide (LDH) wound cover was achieved by preparing a curcumin p-sulfonic acid calix[4]arene (SC4A) inclusion complex. Then, it was utilized to intercalate MgAl-layered double hydroxide (MgAl-LDH) nanosheets. The incorporation of the nanostructure into a PU/Cur-SC4A-LDH film provided bacteria-killing performance against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. This finding is due to an increase in curcumin bioavailability in the PU matrix. Furthermore, all PU nanocomposites exhibited appropriate cytocompatibility based on an MTT assay. Mainly, the proliferation of L929 fibroblast cells in contact with the PU/Cur-SC4A-LDH sample was significantly further enhanced than that for other nanocomposites within 7 days. This observation can be related to the better availability of curcumin on the film's surface, which causes an improvement in the proliferation rate of cells. Regarding the histological results, the hematoxylin and eosin (H&E) images showed faster epidermal layer formation and a larger quantity of matured hair follicles for PU/Cur-SC4A-LDH-healed wounds in comparison with those for the negative control over a period of 28 days. Thus, this practical healing ability of the PU/Cur-SC4A-LDH nanocomposite makes it a promising candidate as a wound dressing film.