RESUMEN
OBJECTIVE: To investigate the utility of urine sampling for detecting human papillomavirus (HPV) DNA among pregnant women and to compare HPV DNA detection in urine with detection in vaginal samples. METHODS: In a cross-sectional study, urine and vaginal samples were self-collected from pregnant women attending prenatal care at Hospital Divina Providencia, Frederico Westphalen, Brazil, between October 2006 and August 2007. Part of the L1 region of the HPV genome was amplified via GP5(+)/bioGP6(+) primers. Positive urine was genotyped for high-risk HPV genotypes (HPV16, HPV18, HPV31, HPV33, HPV39, HPV45, and HPV59). RESULTS: During the study period, urine samples were obtained from 133 pregnant women, 63 of whom also self-collected vaginal samples. HPV DNA was detected in 54.0% (34/63) and 61.9% (39/63) of urine and vaginal samples, respectively. HPV infection was significantly associated with first intercourse at younger than 20 years of age (P=0.008). There was substantial agreement in HPV DNA test results between the urine and vaginal samples (κ value, 77.3%; P<0.0001). HPV31 and HPV16 accounted for 80.7% of the oncogenic types identified. CONCLUSION: Detection of HPV DNA in urine showed good agreement with detection in self-collected vaginal samples, indicating that urine might be a reliable sample for HPV testing among pregnant women.
Asunto(s)
ADN Viral/análisis , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/diagnóstico , Brasil , Estudios Transversales , Femenino , Genotipo , Humanos , Persona de Mediana Edad , Papillomaviridae/genética , Infecciones por Papillomavirus/virología , Embarazo , Reproducibilidad de los Resultados , Autocuidado , Manejo de Especímenes/métodosRESUMEN
This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
Asunto(s)
Humanos , Femenino , Chancro/genética , Chancro/patología , Técnicas In Vitro , Neoplasias del Cuello Uterino/genética , Reacción en Cadena de la Polimerasa/métodos , Diagnóstico , Incidencia , Métodos , Pacientes Ambulatorios , Guías como Asunto , MétodosRESUMEN
This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.