RESUMEN
Cyclophosphamide (CY), the agent with cytoreductive activity, is widely exploited in cancer chemotherapy, and can be used alone or in combination with various cytokines and growth factors to stimulate the egress of hematopoietic stem/progenitor cells (HSPC) from the BM compartment. The aim of the present study was to exam the morphology and ultrastructure of the bone marrow, spleen and liver of mice injected intraperitoneally with a single dose of cyclophosphamide (200 mg/kg bw) and the localization of cells expressing markers of early hematopoietic cells in studied organs and the peripheral blood. We observed that the CY-induced morphological changes in the BM and spleen were reconstructed on day 4. of experiment, and the spleen was repopulated by HSPC on the 6th day. In this time, the highest number of c-Kit-R-positive cells was determined by flow cytometry in the peripheral blood. The results confirmed, that the egress of HSPC from the bone marrow into the peripheral blood was delayed compared to mice treated with G-CSF or GCS-F plus CY.
Asunto(s)
Médula Ósea/efectos de los fármacos , Ciclofosfamida/farmacología , Movilización de Célula Madre Hematopoyética , Hígado/citología , Hígado/efectos de los fármacos , Bazo/citología , Bazo/efectos de los fármacos , Animales , Células Sanguíneas/citología , Células Sanguíneas/efectos de los fármacos , Células Sanguíneas/metabolismo , Médula Ósea/ultraestructura , Células de la Médula Ósea/citología , Células de la Médula Ósea/efectos de los fármacos , Forma de la Célula , Ratones , Ratones Endogámicos BALB C , Proteínas Proto-Oncogénicas c-kit/metabolismo , Reticulina/efectos de los fármacos , Antígenos Thy-1/metabolismoRESUMEN
The aim of the work was to examine the morphology of the bone marrow of mice during stimulation with G-CSF. Experimental Balb C mice were daily injected subcutaneously with 250 microg/kg b.w. G-CSF (Neupogen). After 2, 4 and 6 days of the experiment femurs were obtained for morphological study. On day 2 of the mobilization the amount of haematopoietic cells in the bone marrow increased and dilatation of the sinusoids was observed. Only single leukocytes were observed in the lumen of the vessels. There were numerous leukocytes in the lumen of the sinusoids on day 4 of the mobilization. The morphology of the bone marrow on day 6 was similar to that of the control. Mobilization of mice with G-CSF resulted in migration of haematopoietic cells from the bone marrow and the process is most pronounced on day 4.