RESUMEN
Natural killer T (NKT) cells play a central role in the interface between innate and adaptive immunity, and alpha-galactosylceramide was recently shown to be an endogenous antigen for these cells. The source of alpha-galactosylceramide has not yet been determined; however, in vivo degradation of alpha-galactosylceramide involves generation of alpha-psychosine (alpha-galactosylsphingosine). Alpha-psychosine stimulates cytokine release from NKT cells and constitutes an endogenous antigen for these cells. Alpha-psychosine contains a single lipid chain, while most antigens for NKT cells have two lipid chains, and we have investigated if other glycolipids with one lipid chain, derived from know antigens for NKT cells, stimulate cytokine release from NKT cells. Only psychosine variants derived from the most potent NKT cell antigens cause stimulation, and this stimulation occurs in vitro as well as in vivo. Truncated forms of weak antigens for NKT cells are not stimulatory.
RESUMEN
Due to the prevalence of oligo- and polysaccharides on the surfaces of pathogenic organisms, carbohydrates are primary targets for recognition by antibodies generated by the immune systems of higher organisms. Consequently, substantial effort has been expended in efforts to develop vaccines based on carbohydrate epitopes. Typical approaches involve multivalent presentation of carbohydrate targets on antigenic peptides or proteins, which often involve substantial synthetic commitments and/or vaccines that are heterogeneous and difficult to characterize. We have developed a simple, liposome-based approach to generate multivalent carbohydrate vaccines, and in place of an antigenic peptide or protein, we have used a potent antigen for natural killer T cells. This vaccine, based on the Streptococcus pneumoniae serotype 14 polysaccharide, gave a response superior to that from a clinically used vaccine (Prevnar). The dependence of this response on liposome formation was demonstrated by comparison to a simple mixture of the oligosaccharide and the natural killer T cell adjuvant. The importance of the strength of the adjuvant was observed by use of a potent synthetic adjuvant and a weaker, bacterial derived glycolipid adjuvant. These results demonstrate the effectiveness of this novel and relatively simple means of generating carbohydrate-based vaccines.
RESUMEN
NK cells are required for early control of murine CMV (MCMV) infection, but the distribution of murine NK cells in situ has not been clearly defined. We tested the reactivity of all available NK cell receptor-specific mAbs by immunohistochemistry. Only one mAb, 4D11 (anti-Ly-49G2), was reactive with C57BL/6 tissue sections. mAb 4D11-reactive cells expressed the nuclear morphology and flow cytometric profile of NK cells. In lymphoid organs, NK cells were distributed primarily in the splenic red pulp, between adjacent lobes in lymph node and randomly in the cortex and medulla of the thymus. No NK cells were detected in normal liver sections. Two days following MCMV infection, most splenic NK cells were associated with the lymphoid follicles and marginal zone. By day 3 following infection, the number of liver NK cells had increased significantly and the cells were detected within inflammatory foci. These changes were independent of IL-12, IFN-gamma, and TNF-alpha, as assessed in mice with targeted mutations. Concurrent immunostaining for NK cells and viral Ags revealed close association of NK cells and MCMV-infected cells in the spleen and liver. Similar results were obtained in CD1(-/-) and recombination activation gene-1(-/-) mice lacking NK T or T and B cells, respectively, indicating specificity of staining for NK cells. Thus, following MCMV infection, NK cells accumulate at sites of viral replication in an IL-12-, IFN-gamma-, and TNF-alpha-independent manner.
Asunto(s)
Infecciones por Herpesviridae/inmunología , Células Asesinas Naturales/inmunología , Muromegalovirus , Animales , Antígenos/análisis , Antígenos de Superficie , Complejo CD3/análisis , Interferón gamma/fisiología , Interleucina-12/fisiología , Lectinas Tipo C , Recuento de Leucocitos , Ratones , Ratones Endogámicos C57BL , Subfamilia B de Receptores Similares a Lectina de Células NK , Proteínas/análisis , Factor de Necrosis Tumoral alfa/fisiologíaRESUMEN
Given the broad expression of H-2 class Ib molecules on hematopoietic cells, antigen presentation pathways among CD1d expressing cells might tightly regulate CD1d-restricted natural killer T (NKT) cells. Bone marrow-derived dendritic cells (BM-DCs) and not adherent splenocytes become capable of triggering NK1.1(+)/T cell receptor (TCR)(int) hepatic NKT cell activation when (a) immature BM-DCs lack H-2D(b)-/- molecules or (b) BM-DCs undergo a stress signal of activation. In such conditions, BM-DCs promote T helper type 1 predominant CD1d-restricted NKT cell stimulation. H-2 class Ia-mediated inhibition involves more the direct H-2D(b) presentation than the indirect Qa-1(b) pathway. Such inhibition can be overruled by B7/CD28 interactions and marginally by CD40/CD40L or interleukin 12. These data point to a unique regulatory role of DCs in NKT cell innate immune responses and suggest that H-2 class Ia and Ib pathways differentially control NKT cell recognition of DC antigens.
Asunto(s)
Antígenos CD1/inmunología , Antígeno B7-1/inmunología , Células Dendríticas/inmunología , Antígenos H-2/inmunología , Interferón gamma/biosíntesis , Células Asesinas Naturales/inmunología , Linfocitos T/inmunología , Animales , Antígenos/genética , Antígenos/inmunología , Antígenos CD1/genética , Antígenos CD1d , Antígenos Ly , Antígenos de Superficie , Células de la Médula Ósea/citología , Células de la Médula Ósea/inmunología , Antígenos CD28/inmunología , Diferenciación Celular , Células Cultivadas , Células Dendríticas/citología , Femenino , Antígenos H-2/genética , Antígeno de Histocompatibilidad H-2D , Lectinas Tipo C , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Subfamilia B de Receptores Similares a Lectina de Células NK , Proteínas/genética , Proteínas/inmunología , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Transducción de Señal/inmunologíaRESUMEN
When mutations that inactivate molecules that function in the immune system have been crossed to murine lupus strains, the result has generally been a uniform up-regulation or down-regulation of autoimmune disease in the end organs. In the current work we report an interesting dissociation of target organ disease in beta(2)-microglobulin (beta(2)m)-deficient MRL-Fas(lpr) (MRL/lpr) mice: lupus skin lesions are accelerated, whereas nephritis is ameliorated. beta(2)m deficiency affects the expression of classical and nonclassical MHC molecules and thus prevents the normal development of CD8- as well as CD1-dependent NK1(+) T cells. To further define the mechanism by which beta(2)m deficiency accelerates skin disease, we studied CD1-deficient MRL/lpr mice. These mice do not have accelerated skin disease, excluding a CD1 or NK1(+) T cell-dependent mechanism of beta(2)m deficiency. The data indicate that the regulation of systemic disease is not solely governed by regulation of initial activation of autoreactive lymphocytes in secondary lymphoid tissue, as this is equally relevant to renal and skin diseases. Rather, regulation of autoimmunity can also occur at the target organ level, explaining the divergence of disease in skin and kidney in beta(2)m-deficient mice.
Asunto(s)
Antígenos CD1/metabolismo , Lupus Eritematoso Cutáneo/etiología , Nefritis Lúpica/prevención & control , Microglobulina beta-2/deficiencia , Animales , Antígenos CD1/genética , Femenino , Lupus Eritematoso Cutáneo/inmunología , Lupus Eritematoso Cutáneo/patología , Nefritis Lúpica/inmunología , Nefritis Lúpica/patología , Masculino , Ratones , Ratones Endogámicos MRL lpr , Ratones Noqueados , Especificidad de Órganos , Subgrupos de Linfocitos T/inmunología , Microglobulina beta-2/genéticaRESUMEN
To define the phenotype and T cell receptor (TCR) repertoire of CD1d-dependent T cells, we compared the populations of T cells that persisted in major histocompatibility complex (MHC)-deficient mice, which lack mainstream T cells, with those from MHC/CD1d doubly deficient mice, which lack both mainstream and CD1d-dependent T cells. Surprisingly, up to 80% of the CD1d-dependent T cells were stained by tetramers of CD1d/alpha-galactosylceramide, which specifically identify the previously described CD1d autoreactive Valpha14-Jalpha18/Vbeta8 natural killer (NK) T cells. Furthermore, zooming in on the CD1d-dependent non-Valpha14 T cells, we found that, like Valpha14 NK T cells, they mainly expressed recurrent, CD1d autoreactive TCR families and had a natural memory phenotype. Thus, CD1d-restricted T cells differ profoundly from MHC-peptide-specific T cells by their predominant use of autoreactive and semiinvariant, rather than naive and diverse, TCRs. They more closely resemble other lineages of innate lymphocytes such as B-1 B cells, gammadelta T cells, and NK cells, which express invariant or semiinvariant autoreactive receptors. Finally, we demonstrate that the MHC-restricted TCR repertoire is essentially non-cross-reactive to CD1d. Altogether, these findings imply that lipid recognition by CD1d-restricted T cells may have largely evolved as an innate rather than an adaptive arm of the mouse immune system.
Asunto(s)
Antígenos CD1/inmunología , Linfocitos B/inmunología , Receptores de Antígenos de Linfocitos T/genética , Linfocitos T/inmunología , Animales , Anticuerpos Monoclonales , Antígenos CD1/genética , Antígenos CD1d , Línea Celular , Células Cultivadas , Citotoxicidad Inmunológica , Femenino , Hibridomas/inmunología , Activación de Linfocitos , Complejo Mayor de Histocompatibilidad , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ratas , Receptores de Antígenos de Linfocitos T/análisis , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Bazo/inmunología , TransfecciónRESUMEN
Recently, different members of the CD1 family of MHC-like molecules have been shown to sample different intracellular compartments to present lipid and glycolipid antigens to T cells. Emerging models suggest that CD1 may have evolved to monitor the integrity of membrane lipids and/or to present microbial lipid antigens to both alpha beta and gamma delta T cells.
Asunto(s)
Presentación de Antígeno/inmunología , Antígenos CD1/inmunología , Antígenos CD1/metabolismo , Metabolismo de los Lípidos , Lípidos/inmunología , Transducción de Señal/inmunología , Linfocitos T/inmunología , Linfocitos T/metabolismo , Animales , Humanos , Líquido Intracelular/inmunología , Líquido Intracelular/metabolismo , Líquido Intracelular/microbiología , Linfocitos T/microbiologíaRESUMEN
Endosomal trafficking is an essential component of the CD1 pathway of lipid antigen presentation to T cells. We demonstrate that CD1d access to endosomal compartments is under dual regulation by an intrinsic tyrosine-based motif, which governs intense recycling between the plasma membrane and the endosome, and by the invariant chain, with which CD1d associates in the endoplasmic reticulum. Both pathways independently enhance antigen presentation to V(alpha)14(+) NKT cells, the main subset of CD1d-restricted T cells. These results reveal the complexity of CD1d trafficking and suggest that the invariant chain was a component of ancestral antigen presentation pathways prior to the evolution of MHC and CD1.
Asunto(s)
Presentación de Antígeno/fisiología , Antígenos CD1/metabolismo , Antígenos de Diferenciación de Linfocitos B/fisiología , Retículo Endoplásmico/metabolismo , Endosomas/metabolismo , Antígenos de Histocompatibilidad Clase II/fisiología , Secuencias de Aminoácidos , Animales , Antígenos CD1/química , Antígenos CD1/genética , Antígenos CD1d , Antígenos de Superficie/metabolismo , Linfocitos B/metabolismo , Biotinilación , Membrana Celular/metabolismo , Células Cultivadas/metabolismo , Células Dendríticas/metabolismo , Evolución Molecular , Fibroblastos/metabolismo , Glicosilación , Hibridomas/metabolismo , Cinética , Linfoma de Células B/patología , Lisosomas/metabolismo , Ratones , Microscopía Fluorescente , Unión Proteica , Procesamiento Proteico-Postraduccional , Transporte de Proteínas , Proteínas Recombinantes de Fusión/metabolismo , Fracciones Subcelulares/metabolismo , Transfección , Células Tumorales Cultivadas/metabolismo , Tirosina/químicaRESUMEN
Innate B and T lymphocytes are a subset of lymphocytes that express a restricted set of semi-invariant, germ-line-encoded, autoreactive antigen receptors. Although they have long been set apart from mainstream immunological thought, they now seem to represent a distinct immune-recognition strategy that targets conserved stress-induced self-structures, rather than variable foreign antigens. Innate lymphocytes regulate a range of infectious, tumour and autoimmune conditions. New studies have shed light on the principles and mechanisms that drive their unique development and function, and show their resemblance to another subset of innate lymphocytes, the natural killer cells.
Asunto(s)
Autoinmunidad , Subgrupos de Linfocitos B/inmunología , Subgrupos de Linfocitos T/inmunología , Animales , Autoantígenos , Humanos , Modelos Inmunológicos , Receptores de Antígenos de Linfocitos B/metabolismo , Receptores de Antígenos de Linfocitos T/metabolismo , AutotoleranciaRESUMEN
As a consequence of the peptide specificity of intrathymic positive selection, mice transgenic for a rearranged TCR beta-chain derived from conventional alphabeta T lymphocytes frequently carry mature T cells with significant skewing in the repertoire of the companion alpha-chain. To assess the generality of such an influence, we generated transgenic (Tg) mice expressing a beta-chain derived from nonclassical, NK1.1+ alphabeta T cells, the thymus-derived, CD1. 1-specific DN32H6 T cell hybridoma. Results of the sequence analysis of genomic DNA from developing DN32H6 beta Tg thymocytes revealed that the frequency of the parental alpha-chain sequence, in this instance the Valpha14-Jalpha281 canonical alpha-chain, is specifically and in a CD1.1-dependent manner, increased in the postselection thymocyte population. In accordance, we found phenotypic and functional evidence for an increased frequency of thymic, but interestingly not peripheral, NK1.1+ alphabeta T cells in DN32H6 beta Tg mice, possibly indicating a thymic determinant-dependent maintenance. Thus, in vivo expression of the rearranged TCR beta-chain from a thymus-derived NK1.1+ Valpha14+ T cell hybridoma promotes positive selection of thymic NK1.1+ alphabeta T cells. These observations indicate that the strong influence of productive beta-chain rearrangements on the TCR sequence and specificity of developing thymocytes, which operates through positive selection on self-determinants, applies to both classical and nonclassical alphabeta T cells and therefore represents a general phenomenon in intrathymic alphabeta T lymphocyte development.
Asunto(s)
Antígenos/biosíntesis , Genes Codificadores de la Cadena beta de los Receptores de Linfocito T/inmunología , Biosíntesis de Proteínas , Proteínas , Receptores de Antígenos de Linfocitos T alfa-beta/genética , Subgrupos de Linfocitos T/inmunología , Subgrupos de Linfocitos T/metabolismo , Timo/inmunología , Timo/metabolismo , Transgenes/inmunología , Animales , Antígenos CD1/biosíntesis , Antígenos CD1/metabolismo , Antígenos Ly , Antígenos de Superficie , Diferenciación Celular/genética , Diferenciación Celular/inmunología , Epítopos de Linfocito T/inmunología , Epítopos de Linfocito T/metabolismo , Regulación de la Expresión Génica/inmunología , Reordenamiento Génico de la Cadena alfa de los Receptores de Antígenos de los Linfocitos T , Células Asesinas Naturales/metabolismo , Lectinas Tipo C , Ligandos , Recuento de Linfocitos , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Subfamilia B de Receptores Similares a Lectina de Células NK , Receptores de Antígenos de Linfocitos T alfa-beta/biosíntesis , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Subgrupos de Linfocitos T/citología , Timo/citologíaRESUMEN
CD1, a conserved family of major histocompatibility (MHC)-like glycoproteins in mammals, specializes in capturing lipid rather than peptide antigen for presentation to T lymphocytes. The principles and mechanisms of this newly discovered immune strategy differ markedly from those governing classical MHC-peptide presentation. They might be exploited for the design of new lipid-based microbial vaccines and adjuvants.
Asunto(s)
Antígenos CD1/inmunología , Infecciones/inmunología , Linfocitos T/inmunología , Animales , Humanos , Infecciones/microbiología , Células Asesinas Naturales/inmunología , Lípidos/inmunología , Vacunas/inmunologíaRESUMEN
In this study, we compared the course of a low-dose aerosol Mycobacterium tuberculosis infection in mice bearing gene disruptions for the beta2-microglobulin molecule, the CD8 molecule, and the CD1 molecule. Over the first 50 d of infection, the CD8- and CD1-disrupted mice were no more susceptible to infection than were the control mice. In contrast, the bacterial load in beta2-microglobulin gene-disrupted mice increased rapidly and attained much higher levels than that observed in the other gene-disrupted mice and in control mice. A second major difference between the beta2-microglobulin gene-disrupted mice and the other animals was the development of lung granulomas; both the CD8- and CD1-disrupted mice developed essentially normal granulomas except for an apparent increased lymphocyte influx in the CD8-disrupted mice. The beta2-microglobulin gene-disrupted mice, on the other hand, developed granulomas virtually devoid of lymphocytes, with these cells instead localized within prominent perivascular cuffing adjacent to the lesions. These data support the hypothesis that a beta2-microglobulin-dependent, non-CD8- and non-CD1-dependent mechanism controls the early and efficient influx of protective lymphocytes into infected lesions, and that the absence of this mechanism decreases the capacity of the animal to initially deal with pulmonary tuberculosis.
Asunto(s)
Linfocitos/inmunología , Tuberculosis Pulmonar/inmunología , Microglobulina beta-2/fisiología , Animales , Antígenos CD1/genética , Antígenos CD1/inmunología , Antígenos CD8/genética , Quimiocina CCL2/genética , Quimiocina CCL2/metabolismo , Femenino , Interferón gamma/farmacología , Linfocitos/citología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos , Ratones Noqueados , Mycobacterium tuberculosis/crecimiento & desarrollo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Tuberculosis Pulmonar/microbiología , Microglobulina beta-2/genéticaRESUMEN
Natural killer T (NKT) cells are a unique population of lymphocytes that coexpress a semiinvariant T cell and natural killer cell receptors, which are particularly abundant in the liver. To investigate the possible effect of these cells on the development of the liver stages of malaria parasites, a glycolipid, alpha-galactosylceramide (alpha-GalCer), known to selectively activate Valpha14 NKT cells in the context of CD1d molecules, was administered to sporozoite-inoculated mice. The administration of alpha-GalCer resulted in rapid, strong antimalaria activity, inhibiting the development of the intrahepatocytic stages of the rodent malaria parasites Plasmodium yoelii and Plasmodium berghei. The antimalaria activity mediated by alpha-GalCer is stage-specific, since the course of blood-stage-induced infection was not inhibited by administration of this glycolipid. Furthermore, it was determined that IFN-gamma is essential for the antimalaria activity mediated by the glycolipid. Taken together, our results provide the clear evidence that NKT cells can mediate protection against an intracellular microbial infection.
Asunto(s)
Antimaláricos/farmacología , Galactosilceramidas/farmacología , Células Asesinas Naturales/inmunología , Activación de Linfocitos/efectos de los fármacos , Malaria/inmunología , Plasmodium berghei/inmunología , Plasmodium yoelii/inmunología , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Linfocitos T/inmunología , Animales , Antígenos CD1/inmunología , Antimaláricos/administración & dosificación , Galactosilceramidas/administración & dosificación , Interferón gamma/inmunología , Interleucina-12/inmunología , Células Asesinas Naturales/efectos de los fármacos , Hígado/parasitología , Activación de Linfocitos/inmunología , Malaria/parasitología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Plasmodium berghei/efectos de los fármacos , Plasmodium yoelii/efectos de los fármacos , Linfocitos T/efectos de los fármacosRESUMEN
The CD1 family of major histocompatibility complex (MHC)-like molecules specializes in presenting lipid and glycolipid antigens to alpha/beta T lymphocytes, but little is known about the size of the CD1-restricted T cell population or the frequency of T lymphocytes specific for a given glycolipid antigen. Here, we report the generation and use of mouse CD1d1-glycolipid tetramers to visualize CD1d-restricted T cells. In contrast with previous BIAcore-based estimates of very short half-lives for CD1d-glycolipid complexes, we found that the dissociation rate of several different CD1d-glycolipid complexes was very slow. Fluorescent tetramers of mouse CD1d1 complexed with alpha-galactosylceramide (alphaGalCer), the antigen recognized by mouse Valpha14-Jalpha281/Vbeta8 and human Valpha24-JalphaQ/Vbeta11 natural killer T (NKT) cell T cell receptors (TCRs), allowed us for the first time to accurately describe, based on TCR specificity, the entire population of NKT cells in vivo and to identify a previously unrecognized population of NK1.1-negative "NKT" cells, which expressed a different pattern of integrins. In contrast, natural killer (NK) cells failed to bind the tetramers either empty or loaded with alphaGalCer, suggesting the absence of a CD1d-specific, antigen-nonspecific NK receptor. Mouse CD1d1-alphaGalCer tetramers also stained human NKT cells, indicating that they will be useful for probing a range of mouse and human conditions such as insulin-dependent diabetes mellitus, tumor rejection, and infectious diseases where NKT cells play an important role.
Asunto(s)
Antígenos CD1/inmunología , Antígenos/inmunología , Galactosilceramidas/inmunología , Células Asesinas Naturales/inmunología , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Animales , Antígenos CD1d , Células Cultivadas , Femenino , Colorantes Fluorescentes , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Coloración y Etiquetado/métodos , Linfocitos T/inmunologíaRESUMEN
Biochemical analysis has suggested that self GPI anchors are the main natural ligand associated with mouse CD1d molecules. A recent study reported that Valpha14+ NK T cells responded to self as well as foreign (parasite-derived) GPIs in a CD1d-dependent manner. It further reported that the IgG response to the Plasmodium berghei malarial circumsporozoite (CS) protein was severely impaired in CD1d-deficient mice, leading to a model whereby NK T cells, upon recognition of CD1d molecules presenting the CS-derived GPI anchor, provide help for B cells secreting anti-CS Abs. We tested this model by comparing the anti-CS Ab responses of wild-type, CD1d-deficient, and MHC class II-deficient mice. We found that the IgG response to the CS protein was solely MHC class II-dependent. Furthermore, by measuring the response of a broad panel of CD1d-autoreactive T cells to GPI-deficient CD1d-expressing cells, we found that GPIs were not required for autoreactive responses.
Asunto(s)
Anticuerpos Antiprotozoarios/biosíntesis , Antígenos CD1/fisiología , Glicosilfosfatidilinositoles/fisiología , Antígenos de Histocompatibilidad Clase II/fisiología , Inmunoglobulina G/biosíntesis , Células Asesinas Naturales/inmunología , Activación de Linfocitos/inmunología , Plasmodium berghei/inmunología , Proteínas Protozoarias/inmunología , Subgrupos de Linfocitos T/inmunología , Animales , Antígenos CD1/biosíntesis , Antígenos CD1/genética , Línea Celular , Femenino , Glicosilfosfatidilinositoles/deficiencia , Glicosilfosfatidilinositoles/genética , Hibridomas/inmunología , Hibridomas/metabolismo , Células Asesinas Naturales/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones Transgénicos , Receptores de Antígenos de Linfocitos T alfa-beta/genética , Subgrupos de Linfocitos T/metabolismoRESUMEN
The expression pattern of mouse CD1d and the tissue distribution of CD1d-restricted Valpha14-Jalpha281 NKT cells suggest that the liver and the marginal zone of the spleen might be preferred sites of activation of this potent innate pathway of early cytokine secretion. Because these tissues are particularly involved with the filtration of blood-borne pathogens, and because NKT cells with an activated / memory phenotype accumulate over the first weeks of life and their CD1 ligands bind microbial glycolipids, it has been hypothesized that expansion of the NKT cell subset may be driven by exposure to the microbial environment. To test this hypothesis, we analyzed the frequency, surface phenotype and functional properties of NKT cells in normal and in germ-free C57BL / 6 mice. Surprisingly, we found that the NKT cell subset develops in the presence or absence of a microbial environment. Although these results do not rule out the possibility that NKT cells exert a protective function against some microbial agents, they demonstrate that non microbial ligands, possibly self-antigens are sufficient for the generation, maturation and peripheral accumulation of NKT cells.
Asunto(s)
Vida Libre de Gérmenes/inmunología , Células Asesinas Naturales/inmunología , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Animales , Antígenos CD1/inmunología , Región Variable de Inmunoglobulina/inmunología , Ratones , Ratones Endogámicos C57BL , Especificidad de ÓrganosRESUMEN
Mouse V alpha 14 T cells and their human homologs, V alpha 24 T cells, are prominent subsets of CD1d-restricted T cells. Here we discuss their striking similarities to B-1 B cells and gammadelta T cells and propose that these immune cells mediate various innate strategies in response to endogenous or exogenous danger signals.
Asunto(s)
Antígenos CD1/inmunología , Receptores de Antígenos de Linfocitos T alfa-beta/inmunología , Subgrupos de Linfocitos T/metabolismo , Animales , Humanos , Inmunidad Innata , Células Asesinas Naturales/inmunología , Células Asesinas Naturales/metabolismo , Ratones , Subgrupos de Linfocitos T/inmunologíaRESUMEN
Both thymic and extrathymic bone marrow (BM)-derived pathways for the development of CD1 reactive, Valpha14-Jalpha281(+) NK1.1(+) T cells have been suggested. In this report, we sought evidence for extrathymic NK-T cell development using two approaches. First, BM cells from gammac-deficient mice were examined for the presence of Valpha14-Jalpha281 transcripts. Since intrathymic NK-T cell selection is gammac independent, we predicted that gammac(-) BM cells should also harbor these specific TCRalpha chains. Second, Valpha14-Jalpha281 transcripts were analyzed in BM cells from lethally irradiated, thymectomized mice reconstituted with fetal liver hematopoietic precursors. All donor-derived T cell development in these chimeras is by definition extrathymic. In both cases, we failed to detect invariant Valpha14(+) TCRalpha chain transcripts. These experiments call into question the significance of an extrathymic pathway of development for Valpha14(+) NK1.1(+) CD1-reactive T cells.
Asunto(s)
Antígenos de Diferenciación de Linfocitos B/inmunología , Genes Codificadores de la Cadena alfa de los Receptores de Linfocito T/inmunología , Antígenos de Histocompatibilidad Clase II/inmunología , Células Asesinas Naturales/inmunología , Receptores de Antígenos de Linfocitos T alfa-beta/biosíntesis , Timo/inmunología , Animales , Antígenos de Diferenciación de Linfocitos B/biosíntesis , Antígenos de Diferenciación de Linfocitos B/genética , Diferenciación Celular/inmunología , Feto , Antígenos de Histocompatibilidad Clase II/biosíntesis , Antígenos de Histocompatibilidad Clase II/genética , Células Asesinas Naturales/citología , Ratones , Ratones Endogámicos C57BL , ARN Mensajero/biosíntesis , Quimera por Radiación , TimectomíaRESUMEN
alpha-Galactosylceramide (alpha-GalCer) is a glycolipid with potent antitumor properties that binds to CD1d molecules and activates mouse Valpha14 and human Valpha24 NKT cells. Surprisingly, we found that, as early as 90 min after alpha-GalCer injection in vivo, NK cells also displayed considerable signs of activation, including IFN-gamma production and CD69 induction. NK activation was not observed in RAG- or CD1-deficient mice, and it was decreased by pretreatment with anti-IFN-gamma Abs, suggesting that, despite its rapid induction, it was a secondary event that depended on IFN-gamma release by NKT cells. At later time points, B cells and CD8 T cells also began to express CD69. These findings identify a high-speed communication network between the innate and adaptive immune systems in vivo that is initiated upon NKT cell activation. They also suggest that the antitumor effects of alpha-GalCer result from the sequential recruitment of distinct innate and adaptive effector lymphocytes.
Asunto(s)
Comunicación Celular/inmunología , Células Asesinas Naturales/inmunología , Activación de Linfocitos/inmunología , Subgrupos de Linfocitos T/inmunología , Animales , Antígenos CD/biosíntesis , Antígenos CD1/fisiología , Antígenos de Diferenciación de Linfocitos T/biosíntesis , Galactosilceramidas/administración & dosificación , Galactosilceramidas/inmunología , Inmunidad Innata , Inyecciones Intravenosas , Interferón gamma/biosíntesis , Células Asesinas Naturales/metabolismo , Lectinas Tipo C , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos , Ratones Noqueados , Transducción de Señal/inmunología , Subgrupos de Linfocitos T/metabolismoRESUMEN
Intestinal intraepithelial lymphocytes (IELs) in mice include two main subsets of TCR-alpha/beta(1) cells which differ functionally and ontogenically from each other. One expresses the CD8alpha/alpha homodimer, whereas the other expresses the CD8alpha/beta heterodimer. Although the presence of all CD8(+)TCR-alpha/beta(1) IELs is dependent on beta2-microglobulin molecules, the nature of the major histocompatibility complex (MHC) class I molecules recognized by the CD8alpha/alpha and the CD8alpha/beta(1) subsets has remained elusive. Using mutant mice lacking the expression of both H2-K(b) and H2-D(b), we show that the CD8alpha/beta(1)TCR-alpha/beta(1) subset is dependent on K or D molecules, whereas the CD8alpha/alpha(1)TCR-alpha/beta(1) subset is independent of classical MHC class I molecules. Furthermore, the CD8alpha/alpha(1) cells are conserved in mice lacking expression of CD1, a nonclassical MHC class I-like molecule previously proposed to be a potential ligand for IELs. Using transporter associated with antigen processing (TAP)-deficient mice, this cell population can be further separated into a TAP-dependent and a TAP-independent subset, suggesting either the recognition of two nonclassical MHC-like molecules, only one of which is TAP dependent, or the involvement of a single nonclassical MHC-like molecule that is only partially TAP dependent. These findings demonstrate that CD8alpha/beta(1)TCR-alpha/beta(1) IELs are restricted by H-2K and H-2D molecules, whereas the unusual subset of CD8alpha/alpha(1)TCR-alpha/beta(1) resident IELs recognize nonclassical MHC class I-like molecules that are distinct from CD1.