RESUMEN
BACKGROUND: Human polyomavirus JC (JCV) is a widespread human virus with profound pathogenic potential. A study was undertaken to quantify JCV load in urine and peripheral blood samples of immunocompetent, apparently healthy tribal individuals of North-Eastern part of West Bengal, India for the first time. MATERIALS AND METHODS: One hundred and thirteen samples of urine or blood were collected from different tribal groups of this region. For the quantitative estimation of the viral load in each sample, real-time polymerase chain reaction method using the SYBR Green dye was employed. RESULTS: The viral load estimated was found in the range between 3.5 × 102 and 2.12 × 106 copies/ml of samples having a mean and median viral copy numbers of 8.67 × 105 and 9.19 × 105 copies/ml of sample respectively. CONCLUSION: The mean viral DNA load in urine samples of the studied immunocompetent population was found to be higher than that found in a study conducted in the USA, but lower than similar groups of Italy and healthy adult women in the USA. However when compared with median values of viral DNA loads in urine samples of immunocompetent human subjects of Kuwait, Portugal, and Switzerland the observed viral DNA load was found to be substantially higher.
Asunto(s)
Sangre/virología , Portador Sano/virología , Virus JC/aislamiento & purificación , Infecciones por Polyomavirus/virología , Infecciones Tumorales por Virus/virología , Orina/virología , Carga Viral , ADN Viral/genética , ADN Viral/aislamiento & purificación , Femenino , Humanos , India , Virus JC/genética , Masculino , Grupos de Población , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Koro in the background of drug abuse is quite rare. The present report of Koro during the first experience with cannabis is the only account of its kind in the world Koro literature to date. The nature and extent of this genital depersonalization is discussed.